1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. Chymase/CMA1 蛋白, Mouse (His)

Chymase/CMA1 蛋白, Mouse (His)

目录号: HY-P72290
COA 产品使用指南

食糜酶/CMA1 蛋白是肥大细胞中一种重要的分泌蛋白酶,参与多种生理过程,包括血管活性肽的生成、细胞外基质降解和腺体分泌的调节。Chymase/CMA1 蛋白, Mouse (His) 是重组的 食糜酶/CMA1 蛋白,由 E. coli 表达,带有 N-His 标签。Chymase/CMA1 蛋白, Mouse (His) 全长 225 个氨基酸,分子量约为 ~29.2 kDa。

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     可免费申领三个不同产品的试用装。

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规格 价格 是否有货 数量
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2 μg ¥580 In-stock
10 μg ¥1450 In-stock
50 μg ¥3050 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Chymase/CMA1 Protein, a prominent secreted protease in mast cells, is implicated in various physiological processes, including the generation of vasoactive peptides, extracellular matrix degradation, and the regulation of gland secretion. Chymase/CMA1 Protein, Mouse (His) is the recombinant mouse-derived Chymase/CMA1 protein, expressed by E. coli , with N-His labeled tag. The total length of Chymase/CMA1 Protein, Mouse (His) is 225 a.a., with molecular weight of ~29.2 kDa.

研究背景

食糜酶/CMA1 蛋白是肥大细胞中主要的分泌蛋白酶,表明其参与血管活性肽的生成、细胞外基质降解和腺体分泌的调节。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Mouse

表达系统

E. coli

标签

N-His

蛋白编号

P21844 (I22-E246)

基因 ID

17228  [NCBI]

蛋白结构
N-term
His
CMA1 (I22-E246)
Accession # P21844
C-term
同用名
Alpha-chymase; Mast cell chymase 1; Mast cell protease 5; mMCP-5; Mast cell protease I
氨基酸序列

IGGTECIPHSRPYMAYLEIVTSENYLSACSGFLIRRNFVLTAAHCAGRSITVLLGAHNKTSKEDTWQKLEVEKQFLHPKYDENLVVHDIMLLKLKEKAKLTLGVGTLPLSANFNFIPPGRMCRAVGWGRTNVNEPASDTLQEVKMRLQEPQACKHFTSFRHNSQLCVGNPKKMQNVYKGDSGGPLLCAGIAQGIASYVHRNAKPPAVFTRISHYRPWINKILREN

分子量

Approximately 29.2kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4.

内毒素含量

<23 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Chymase/CMA1 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Chymase/CMA1 Protein, Mouse (His)
目录号:
HY-P72290
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