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  4. CK2 alpha/CSNK2A1 蛋白, Mouse (sf9, His-GST)

CK2 alpha/CSNK2A1 蛋白, Mouse (sf9, His-GST)

目录号: HY-P76826
产品使用指南

CK2α/CSNK2A1 蛋白能磷酸化丝氨酸或苏氨酸。它参与细胞周期、凋亡、转录和病毒感染的调控,对纺锤体组装检查点和 p53/TP53 介导的细胞凋亡有保护效应。CK2α/CSNK2A1 还能通过磷酸化各类因子参与信号传导,影响生物钟功能和病毒生命周期。CK2 alpha/CSNK2A1 蛋白, Mouse (sf9, His-GST) 是重组的 CK2 α/CSNK2A1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。CK2 alpha/CSNK2A1 蛋白, Mouse (sf9, His-GST) 全长 391 个氨基酸,分子量约为 ~65 kDa。

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描述

CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CK2 alpha/CSNK2A1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CK2 alpha/CSNK2A1 Protein, Mouse (sf9, His-GST) is 391 a.a., with molecular weight of ~65 kDa.

研究背景

CK2α/CSNK2A1 蛋白的催化亚基是组成型活性丝氨酸/苏氨酸蛋白激酶复合物的组成部分,具有磷酸化磷酸化丝氨酸或苏氨酸 C 端富含酸性残基的众多底物的能力。这种多方面的激酶调节多种细胞过程,包括细胞周期进程、细胞凋亡、转录和病毒感染。作为调节中心,CK2α/CSNK2A1 整合信号并协调适当的细胞反应,例如在有丝分裂期间维持纺锤体组装检查点并促进 p53/TP53 介导的细胞凋亡。此外,CK2 α/CSNK2A1 通过磷酸化各种转录因子、凋亡调节因子和细胞外蛋白参与复杂的信号传导网络。它的影响延伸到生物钟功能和病毒生命周期,强调其在细胞稳态和对外部刺激的反应中的关键作用。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q60737 (M1-Q391)

基因 ID

12995  [NCBI]

蛋白结构
N-term
His-GST
Csnk2a1 (M1-Q391)
Accession # Q60737
C-term
同用名
Casein kinase II subunit alpha; CK II alpha; CSNK2A1; CK2A1
分子量

Approximately 65 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

CK2 alpha/CSNK2A1 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CK2 alpha/CSNK2A1 Protein, Mouse (sf9, His-GST)
目录号:
HY-P76826
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