CRTAM/CD355 蛋白, Human (HEK293, His)

The CRTAM/CD355 protein is a key mediator of heterophilic intercellular adhesion and complexly regulates the activation, differentiation, and tissue retention of T cell subsets. Its interaction with CADM1 promotes NK cell cytotoxicity and CD8+ T cell secretion of IFNG, thus contributing to NK cell-mediated tumor rejection. CRTAM/CD355 Protein, Human (HEK293, His) is the recombinant human-derived CRTAM/CD355 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CRTAM/CD355 Protein, Human (HEK293, His) is 269 a.a., with molecular weight of 58-64 kDa.

CRTAM/CD355 蛋白是异嗜性细胞间粘附的重要介质，复杂地调节各种 T 细胞亚群的激活、分化和组织保留。它与 CADM1 的相互作用在促进自然杀伤 (NK) 细胞的细胞毒性以及体外和体内 CD8+ T 细胞分泌 IFNG/干扰素-γ 方面发挥着关键作用，有助于 NK 细胞介导的对 CADM1 表达的排斥肿瘤。CRTAM 还响应 T 细胞受体 (TCR) 激活调节 CD8+ T 细胞增殖，并通过与 SCRIB 相互作用，促进 CD4+ T 细胞亚群的细胞极化后期，调节 TCR 介导的增殖和细胞因子产生。此外，CRTAM 与 CD8+ 树突状细胞上的 CADM1 相互作用，调节引流淋巴结内活化的 CD8+ T 细胞的保留。CRTAM 对于上皮内 T 细胞亚群的肠道保留至关重要，它进一步促进与肠道相关 CD103+ 树突状细胞的粘附，促进 T 细胞上肠道归巢和粘附分子的表达。作为可能形成同型二聚体的单体，CRTAM 复杂的相互作用，特别是与 CADM1 和 SCRIB 的相互作用，凸显了它在协调对免疫反应和组织稳态至关重要的多种细胞功能中的核心作用。对这些相互作用的进一步探索将揭示 CRTAM 调节功能背后的精确机制。

Human

HEK293

C-6*His

O95727 (S18-S286)

56253  [NCBI]

SLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKS

58-64 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.