DSP 蛋白, Human (His-SUMO)

DSP proteins are major components of desmosomes and are critical for the structural integrity of various tissues. In cardiomyocytes, DSP regulates profibrotic gene expression through MAPK14/p38 MAPK activation and increased TGFB1 protein. DSP Protein, Human (His-SUMO) is the recombinant human-derived DSP protein, expressed by E. coli , with N-6*His, N-SUMO labeled tag. The total length of DSP Protein, Human (His-SUMO) is 223 a.a., with molecular weight of ~42.1 kDa.

DSP 蛋白作为桥粒的主要高分子量成分占据中心地位，对于维持各种组织的结构完整性至关重要。在心肌细胞中，DSP 通过激活 MAPK14/p38 MAPK 信号级联和增加 TGFB1 蛋白丰度在促纤维化基因表达中发挥调节作用，基于相似性的观察证明了这一点。DSP 作为同二聚体存在，与关键桥粒成分（如 COL17A1、DSC2、PKP2 和 PKP1）进行动态相互作用，展示了其在桥粒连接复杂结构中的不可或缺的作用。此外，DSP 与 TMEM65 的相互作用较弱，突显了其在桥粒组装之外的多种细胞过程中的潜在参与。DSP 的多方面功能强调了其在细胞结构和信号通路中的重要性。

Human

E. coli

N-6*His;N-SUMO

P15924 (C78-D300)

1832  [NCBI]

CSDCLMRAELIVQPELKYGDGIQLTRSRELDECFAQANDQMEILDSLIREMRQMGQPCDAYQKRLLQLQEQMRALYKAISVPRVRRASSKGGGGYTCQSGSGWDEFTKHVTSECLGWMRQQRAEMDMVAWGVDLASVEQHINSHRGIHNSIGDYRWQLDKIKADLREKSAIYQLEEEYENLLKASFERMDHLRQLQNIIQATSREIMWINDCEEEELLYDWSD

Approximately 42.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.