2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Elastase IIIB/CELA3B 蛋白, Mouse (Myc, His)

Elastase IIIB/CELA3B 蛋白, Mouse (Myc, His)

目录号: HY-P71638
产品使用指南

弹性蛋白酶 IIIB/CELA3B 蛋白作为蛋白酶表现出显着的效率，显示出对丙氨酸特异性的明显偏好，同时表现出有限的弹力分解活性。这种独特的酶谱使该蛋白质成为精确底物裂解的有希望的候选者，特别是在处理富含丙氨酸的底物时。Elastase IIIB/CELA3B 蛋白, Mouse (Myc, His) 是重组的 弹性蛋白酶 IIIB/CELA3B 蛋白，由 E. coli 表达，带有 N-His, C-Myc 标签。Elastase IIIB/CELA3B 蛋白, Mouse (Myc, His) 全长 242 个氨基酸，分子量约为 ~31.1 kDa。

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描述

The elastase IIIB/CELA3B protein exhibits remarkable efficiency as a protease, showing a clear preference for alanine specificity while exhibiting limited elastolytic activity. This unique zymogram makes this protein a promising candidate for precise substrate cleavage, especially when processing alanine-rich substrates. Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is the recombinant mouse-derived Elastase IIIB/CELA3B protein, expressed by E. coli , with N-His, C-Myc labeled tag. The total length of Elastase IIIB/CELA3B Protein, Mouse (Myc, His) is 242 a.a., with molecular weight of ~31.1 kDa.
研究背景

弹性蛋白酶 IIIB，也称为 CELA3B，是一种有效的蛋白酶，对丙氨酸具有显着的特异性，但仅表现出有限的弹性分解活性。该酶的特点是能够特异性地在丙氨酸残基处裂解肽键，这表明它偏爱含有该氨基酸的底物。虽然它没有表现出很强的弹力分解活性，但其有效的蛋白水解功能表明其在含有丙氨酸残基的蛋白质的靶向裂解中发挥作用。弹性蛋白酶 IIIB/CELA3B 独特的底物特异性强调了其在生物系统内蛋白水解过程中的独特作用。
种属

Mouse
表达系统

E. coli
标签

N-His;C-Myc
蛋白编号

Q9CQ52 (28V-269N)
基因 ID

67868  [NCBI]
蛋白结构
N-term
10*His
CELA3B (28V-269N)
Accession # Q9CQ52
C-term
同用名
Cela3b; Ela3; Ela3b; Chymotrypsin-like elastase family member 3B; EC 3.4.21.70; Elastase IIIB; Elastase-3B; Protease E
氨基酸序列

VVNGEEAVPHSWPWQVSLQYEKDGSFHHTCGGSLITPDWVLTAGHCISTSRTYQVVLGEHERGVEEGQEQVIPINAGDLFVHPKWNSMCVSCGNDIALVKLSRSAQLGDAVQLACLPPAGEILPNGAPCYISGWGRLSTNGPLPDKLQQALLPVVDYEHCSRWNWWGLSVKTTMVCAGGDIQSGCNGDSGGPLNCPADNGTWQVHGVTSFVSSLGCNTLRKPTVFTRVSAFIDWIEETIANN

分子量

Approximately 31.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Elastase IIIB/CELA3B Protein, Mouse (Myc, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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