FNDC4 蛋白, Human (HEK293, Fc)

The FNDC4 protein is an anti-inflammatory factor in the intestine and colon that regulates macrophages by downregulating pro-inflammatory gene expression and affecting phagocytosis. It does this by modulating key pathways associated with macrophage activation, in part through STAT3 activation and signaling. FNDC4 Protein, Human (HEK293, Fc) is the recombinant human-derived FNDC4 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of FNDC4 Protein, Human (HEK293, Fc) is 123 a.a., with molecular weight of ~50-60 KDa.

FNDC4 蛋白在肠道和结肠中充当抗炎因子，对巨噬细胞发挥调节作用。通过与巨噬细胞的结合和相互作用，FNDC4 下调促炎基因的表达，影响巨噬细胞的重要功能，例如吞噬作用。这种抗炎作用是通过调节与巨噬细胞激活相关的关键途径来实现的，部分是通过 STAT3 的激活和信号传导来实现的。FNDC4 在抑制免疫反应中的作用，特别是在结肠炎的情况下，强调了其作为维持肠道免疫稳态的分子介质的潜在重要性。

Measured in a cell proliferation assay using U87 cells. The ED₅₀ for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×10⁴ unit/mg.

• 
  Measured in a cell proliferation assay using U87 cells. The ED₅₀ for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×10⁴ Unit/mg.

Human

HEK293

C-mFc

Q9H6D8/NP_073734.1 (D45-T167)

64838  [NCBI]

DRPPSPVNVTVTHLRANSATVSWDVPEGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRLPSNSSSPGDITVEGLDGERPLQT

Approximately 50-60 kDa due to the glycosylation

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.