Galectin-3/LGALS3 蛋白, Cynomolgus (HEK293, His)

The Galectin-3/LGALS3 protein is a galactose-specific lectin known for its diverse roles in cellular processes. It binds IgE and synergizes with α-3 and β-1 integrins to promote CSPG4-induced endothelial cell migration. Galectin-3/LGALS3 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Galectin-3/LGALS3 protein, expressed by HEK293 , with N-His labeled tag. The total length of Galectin-3/LGALS3 Protein, Cynomolgus (HEK293, His) is 247 a.a., with molecular weight of 35-48 kDa.

Galectin-3/LGALS3 蛋白是一种半乳糖特异性凝集素，以其在细胞过程中的多种作用而闻名。它结合 IgE，并与 α-3、β-1 整合素协同作用，促进 CSPG4 诱导的内皮细胞迁移。与 DMBT1 一起，它对于早期胚胎发生过程中柱状上皮细胞的终末分化至关重要。在细胞核中，该蛋白作为前体 mRNA 剪接因子，积极参与急性炎症反应，影响中性粒细胞活化、粘附、单核细胞和巨噬细胞的化学吸引、凋亡中性粒细胞的调理作用和肥大细胞活化。它与 TRIM16 的合作可以协调识别膜损伤，触发核心自噬调节因子 ATG16L1 和 BECN1 的动员，以应对受损的内膜。该蛋白可能形成同源或异源二聚体，并与各种伙伴结合，包括 DMBT1、CD6、ALCAM、ITGA3、ITGB1、CSPG4、LGALS3BP、LYPD3、ZFTRAF1、UACA、TRIM16 和 TMED10，促进多种细胞相互作用，包括自噬和分泌。

Immobilized Cynomolgus Galectin 3, His Tag at 2 μg/mL (100 μl/Well) on the plate. Dose response curve for Anti-Galectin 3 Antibody, hFc Tag with the EC₅₀ of <38.4 ng/mL determined by ELISA.

Cynomolgus

HEK293

N-His

XP_005561371.1 (A2-I248)

102145569  [NCBI]

35-48 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Hepes, 150 mM NaCl, 200 mM L-Arginine, 2 mM Tcep, pH 6.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.