GCGR/Glucagon receptor 蛋白, Mouse (HEK293, Fc)

GCGR/glucagon receptor protein is a G protein-coupled receptor for glucagon that centrally regulates blood glucose levels. It plays a key role in the fasting response by actively regulating hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. GCGR/Glucagon receptor Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived GCGR/Glucagon receptor protein, expressed by HEK293 , with C-hFc labeled tag. The total length of GCGR/Glucagon receptor Protein, Mouse (HEK293, Fc) is 111 a.a., with molecular weight of 50-65 kDa.

GCGR/胰高血糖素受体蛋白作为胰高血糖素的 G 蛋白偶联受体，在血糖水平和葡萄糖稳态的调节中发挥核心作用。它通过促进糖原水解和糖异生来积极调节肝葡萄糖的产生速率，使其成为禁食反应的关键介质。配体结合后，受体会发生构象变化，通过鸟嘌呤核苷酸结合蛋白（G 蛋白）启动信号传导，随后调节下游效应子（如腺苷酸环化酶）的活性。这种调节导致腺苷酸环化酶激活的促进。此外，该受体通过磷脂酰肌醇-钙第二信使系统参与信号传导，进一步强调了其在葡萄糖调节中的多方面作用。

Mouse

HEK293

C-hFc

Q61606 (A27-K137)

14527  [NCBI]

50-65 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5. Normally trehalose is added as protectant before lyophilization.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.