GDF-9 蛋白, Human (GST)

The GDF-9 protein is critical for ovarian folliculogenesis, promoting primordial follicle development and stimulating granulosa cell proliferation. It induces cell cycle transition, upregulates CCND1 and CCNE1 expression, and phosphorylates RB1. GDF-9 Protein, Human (GST) is the recombinant human-derived GDF-9 protein, expressed by E. coli , with N-GST labeled tag. The total length of GDF-9 Protein, Human (GST) is 135 a.a., with molecular weight of ~42.5 kDa.

GDF-9蛋白对于卵巢卵泡发生至关重要，在促进原始卵泡发育和刺激颗粒细胞增殖方面发挥着关键作用。它通过上调 CCND1 和 CCNE1 的表达以及 RB1 的磷酸化，促进细胞从 G0/G1 向 S 和 G2/M 期的转变。此外，GDF-9 调节颗粒细胞和鞘细胞中的 STAR 表达和 cAMP 依赖性孕酮释放。它通过增加抑制素 B 的表达来抵消激活素 A 对 STAR 表达和孕酮产生的抑制作用。此外，GDF-9 抑制颗粒叶黄素细胞中 FST 和 FSTL3 的产生。在形成同二聚体或异二聚体时，与其他家族成员不同，GDF-9 不能发生二硫键连接的相互作用。

Human

E. coli

N-GST

O60383 (G320-R454)

2661  [NCBI]

QETVSSELKKPLGPASFNLSEYFRQFLLPQNECELHDFRLSFSQLKWDNWIVAPHRYNPRYCKGDCPRAVGHRYGSPVHTMVQNIIYEKLDSSVPRPSCVPAKYSPLSVLTIEPDGSIAYKEYEDMIATKCTCR

Approximately 42.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution in Tris-based buffer, 50% glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.