GM-CSF 蛋白, Rat (CHO)

GM-CSF Protein, Rat (CHO) is a hematopoietic growth factor and immune modulator.

粒细胞巨噬细胞集落刺激因子（GM-CSF）是由多种细胞类型（包括 T 细胞、巨噬细胞、内皮细胞和成纤维细胞）在接受免疫刺激后产生的。它是一种重要的造血生长因子和免疫调节剂。GM-CSF 对各种循环白细胞的功能活动也有深远的影响。GM-CSF根据其浓度刺激多能祖细胞，最低剂量时巨噬细胞祖细胞的增殖，其次是粒细胞、红细胞、嗜酸性粒细胞、巨核细胞和多能祖细胞。在某些情况下，它还能刺激髓系白血病细胞的分化并控制嗜酸性粒细胞功能^[1][2]。GM-CSF 还增强成熟细胞的功能，例如中性粒细胞。在中性粒细胞中，GM-CSF 增强脱颗粒、氧和氮自由基离子的释放、吞噬作用并抑制细胞凋亡^[3]。GM-CSF 抑制在一些自身免疫性疾病动物模型中表现出显着的有益作用^[4]。GM-CSF 还具有许多促炎功能，最近的数据表明 GM-CSF 是 Th17 驱动的自身免疫性炎症的关键因素^[5]。

1.The ED₅₀ is <5 pg/mL as measured by FDC-P1 cells, corresponding to a specific activity of >2 × 10⁸ units/mg.
2.Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 5.019 pg/mL, corresponding to a specific activity is 1.99×10⁸ units/mg.

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  Measured in a cell proliferation assay using M‑NFS‑60 mouse myelogenous leukemia lymphoblast cells. The ED₅₀ for this effect is 5.019 pg/mL, corresponding to a specific activity is 1.99×10⁸ units/mg.

Rat

CHO

Tag Free

P48750-1 (A18-K144)

116630  [NCBI]

APTRSPNPVTRPWKHVDAIKEALSLLNDMRALENEKNEDVDIISNEFSIQRPTCVQTRLKLYKQGLRGNLTKLNGALTMIASHYQTNCPPTPETDCEIEVTTFEDFIKNLKGFLFDIPFDCWKPVQK

16-26 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Shi Y, et al. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and T-cell responses: what we do and don't know. Cell Res. 2006 Feb;16(2):126-33.  [Content Brief]

  [2]. Gasson JC, et al. Human granulocyte-macrophage colony-stimulating factor (GM-CSF): regulation of expression. Prog Clin Biol Res. 1990;338:27-41.  [Content Brief]

  [3]. Gomez-Cambronero J, et al. Granulocyte-macrophage colony-stimulating factor is a chemoattractant cytokine for human neutrophils: involvement of the ribosomal p70 S6 kinase signaling pathway. J Immunol. 2003 Dec 15;171(12):6846-55.  [Content Brief]

  [4]. Shiomi A, et al. GM-CSF as a therapeutic target in autoimmune diseases. Inflamm Regen. 2016 Jul 5;36:8.  [Content Brief]

  [5]. van Nieuwenhuijze A, et al. GM-CSF as a therapeutic target in inflammatory diseases. Mol Immunol. 2013 Dec;56(4):675-82.  [Content Brief]