HLA-E*0103 Complex Tetramer 蛋白, Human (Biotinylated, HEK293, His-Avi)

The HLA-E*0103 Complex, a non-classical major histocompatibility class Ib molecule, is crucial for immune self-nonself discrimination. It selectively binds VL9 peptides from classical MHC class Ia molecules, forming a complex with B2M. This complex acts as a ligand for KLRD1-KLRC1 on NK cells, facilitating immune monitoring. Under stress, HLA-E*0103 binds stress-induced chaperone peptides and HLA-G peptides, influencing NK cell activation and contributing to adaptive NK cell functions during pregnancy. Additionally, it presents pathogen-derived peptides to CD8-positive T cells, triggering an antimicrobial immune response. HLA-E*0103 Complex Tetramer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing HLA-E*0103 and B2M/Beta-2-microglobulin Protein, expressed by HEK293 , with C-Avi, C-His labeled tag and VMAPRTLVL peptide. HLA-E*0103 Complex Tetramer Protein, Human (Biotinylated, HEK293, His-Avi), has molecular weight of 260-265 kDa.

HLA-E*0103 复合物是一种非经典的主要组织相容性 Ib 类分子，在免疫自我-非自我辨别中发挥着关键作用。该分子与 B2M/beta-2-微球蛋白形成复合物，并选择性结合源自经典 MHC Ia 类分子信号序列的九聚体自肽，特别是 VL9 肽 (VMAPRT[V/L][L/V/I/F ]L）。肽结合的 HLA-E-B2M 异三聚体复合物作为自然杀伤 (NK) 细胞上抑制性受体 KLRD1-KLRC1 的配体，使这些细胞能够监测健康细胞中其他 MHC I 类分子的表达并耐受自身。在细胞应激期间，HLA-E*0103 优先结合来自应激诱导伴侣的信号序列衍生肽，导致 NK 细胞的保护受损。此外，它还结合来自非经典 MHC Ib 类 HLA-G 分子的信号序列衍生肽，作为 NK 细胞激活受体 KLRD1-KLRC2 的配体，可能有助于妊娠期间的适应性 NK 细胞功能和母胎耐受。此外，HLA-E*0103 可以结合并将病原体衍生的肽呈递给非常规 CD8 阳性细胞毒性 T 细胞上的 α-β T 细胞受体，从而引发抗菌免疫反应。该分子还呈现来自人类巨细胞病毒等病毒的特定肽，促进对受感染细胞的免疫耐受。

Human

HEK293

C-Avi;C-His

P13747 (G25-I305)&P61769 (I21-M119)&VMAPRTLVL

3133  [NCBI]&567  [NCBI]

260-265 kDa

Greater than 95% as determined by Tris-Bis PAGE.

<1 EU/μg, determined by LAL method.