1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. hydG 蛋白, Pyrococcus furiosus (His)

hydG 蛋白, Pyrococcus furiosus (His)

目录号: HY-P701919
产品使用指南

HydG 是双功能酶复合物的关键成分,作为 NADPH 依赖性析氢氢化酶发挥硫还原活性。它在发酵生长过程中氢循环中的作用凸显了它的细胞重要性。hydG 蛋白, Pyrococcus furiosus (His) 是重组的 hydG 蛋白,由 E. coli 表达,带有 N-6*His 标签。hydG 蛋白, Pyrococcus furiosus (His) 全长 292 个氨基酸。

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描述

HydG is a key component of a bifunctional enzyme complex that exerts sulfur reducing activity as an NADPH-dependent hydrogen evolution hydrogenase. Its role in hydrogen cycling during fermentative growth highlights its cellular importance. hydG Protein, Pyrococcus furiosus (His) is the recombinant hydG protein, expressed by E. coli , with N-6*His labeled tag. The total length of hydG Protein, Pyrococcus furiosus (His) is 292 a.a., .

研究背景

HydG 是双功能酶复合物的重要组成部分,作为具有硫还原活性的 NADPH 依赖性析氢氢化酶发挥作用。它在发酵生长过程中参与氢循环的潜在参与强调了它在细胞过程中的重要性。有趣的是,HydG 的活性对于 NAD 并不明显,它的 β 和 γ 亚基的协同作用形成了硫还原成分,在元素硫存在的情况下促进细胞质产生硫化氢。值得注意的是,当暴露于硫酸钠、亚硫酸钠、硫代硫酸钠或半胱氨酸时,HydG 仍然不活跃,这揭示了它对环境因素的微妙反应。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

Q8U2E4 (M1-D292)

基因 ID

41712700

蛋白结构
N-term
6*His
hydG (M1-D292)
Accession # Q8U2E4
C-term
同用名
hydG; Sulfhydrogenase 1 subunit gamma; Sulfhydrogenase I subunit gamma; Sulfur reductase subunit HydG
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

hydG Protein, Pyrococcus furiosus (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
hydG Protein, Pyrococcus furiosus (His)
目录号:
HY-P701919
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