IGFBP-2 蛋白, Human (HEK293, His)

IGFBP2 Protein, Human (HEK293, His) is a member of the family of high-affinity binding proteins (IGFBP1-6) and appears to play a governing role in Insulin-like Growth Factor (IGF) regulation in the central nervous system.

胰岛素样生长因子结合蛋白 2 (IGFBP2) 是高亲和力结合蛋白 (IGFBP1-6) 家族的成员，似乎在中枢神经系统的胰岛素样生长因子 (IGF) 调节中发挥控制作用^[1]。胰岛素样生长因子结合蛋白 (IGFBP) 通过与分泌的胰岛素样生长因子 (IGF) 结合来调节其作用，并通过以这种结合形式隔离 IGF 来延长 IGF 在细胞外环境和循环中的半衰期。IGFBP 增强或抑制 IGF 对靶细胞的作用；单个 IGFBP 可以抑制或增强 IGF 对骨中成骨细胞的作用。人胰岛素样生长因子结合蛋白 5 (rhIGFBP-5) 是骨骼中储存的一种独特且最丰富的 IGFBP，对羟基磷灰石和细胞外基质蛋白具有高特异性结合亲和力，因此将其及其结合的 IGF 固定在骨骼内。rhIGFBP-5 在体外持续刺激成骨细胞增殖，从而增加成骨细胞的数量^[2]。

1. The ED₅₀ is <2 µg/mL as measured by FDC-P1 cells.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1501 μg/ml in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 6.662×10³ units/mg.

• 
  Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1501 μg/ml in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 6.662×10³ units/mg.

Human

HEK293

C-His

P18065 (F40-Q325)

3485  [NCBI]

FRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACGVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQHHHHHH

Approximately 34.26 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Milanesi E, et al. Insulin-like growth factor binding protein 2 in bipolar disorder: An expression study in peripheral tissues. World J Biol Psychiatry. 2018 Dec;19(8):610-618.  [Content Brief]

  [2]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705.  [Content Brief]