1. 重组蛋白
  2. Cytokines and Growth Factors
  3. IGF family
  4. IGFBP-3
  5. IGFBP-3 蛋白, Human

IGFBP-3 蛋白, Human

目录号: HY-P7020
COA 产品使用指南

IGFBP-3 蛋白, Human属于胰岛素样生长因子结合蛋白家族,能够延长胰岛素样生长因子的半衰期,阻止胰岛素样生长因子与其细胞表面受体的相互作用,同时可以降低癌症的发生。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1250 In-stock
50 μg ¥3500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

IGFBP-3 Protein, Human belongs to insulin-like growth factor-binding protein, prolongs the half-life of the insulin-like growth factors (IGFs), inhibits interaction of IGFs with their cell surface receptors, and functions in cancer reduction.

研究背景

胰岛素样生长因子结合蛋白 (IGFBP) 通过与分泌的胰岛素样生长因子 (IGF) 结合来调节其作用,并通过以这种结合形式隔离 IGF 来延长 IGF 在细胞外环境和循环中的半衰期。IGFBP 增强或抑制 IGF 对靶细胞的作用;各个 IGFBP 会抑制或增强 IGF 对骨中成骨细胞的作用[1]。胰岛素样生长因子结合蛋白 3 (IGFBP3) 参与延长 IGF 的半衰期(从 12 分钟至 12 小时),抑制 IGF 与其细胞表面受体的相互作用,并且它是一种循环蛋白IGF 的储存库。此外,IGFBP3 通过 IGF 依赖性和 IGF 独立机制发挥减少癌症的作用。研究表明,高剂量的 IGFBP3 可以降低患乳腺癌的风险。高浓度的 IGFBP3 可阻止 IGF1:IGF1R 相互作用并阻断 IGF1R 酪氨酸激酶活性,从而抑制细胞内信号级联的形成[2]

生物活性

The ED50 is <200 ng/mL as measured by serum free human MCF-7 cells, corresponding to a specific activity of >5.0 × 103 units/mg.

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P17936 (G28-K291)

基因 ID
蛋白结构
N-term
IGFBP-3 (G28-K291)
Accession # P17936
C-term
中文名
重组人胰岛素样生长因子结合蛋白 3
同用名
rHuIGF-BP3; IBP-3; IGF-binding protein 3; IGFBP-3
氨基酸序列

GASSGGLGPVVRCEPCDARALAQCAPPPAVCAELVREPGCGCCLTCALSEGQPCGIYTERCGSGLRCQPSPDEARPLQALLDGRGLCVNASAVSRLRAYLLPAPPAPGNASESEEDRSAGEVESPSVSSTHRVSDPKFHPLHSKIIIIKKGHAKDSQRYKVDYESQSTDTQNFSSESKRETEYGPCRREMEDTLNHLKFLNVLSPRGVHIPNCDKKGFYKKKQCRPSKGRKR

分子量

Approximately 28.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IGFBP-3 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
IGFBP-3 Protein, Human
目录号:
HY-P7020
需求量: