1. 重组蛋白
  2. Fc Receptors
  3. Immunoglobulin Fc Region
  4. IgG2A
  5. IgG2A Fc 蛋白, Rat (HEK293)

IgG2A Fc 蛋白, Rat (HEK293)

目录号: HY-P72604
COA 产品使用指南

Ig gamma-2A 链 C 区 (IgG2a) 是 IgG 抗体亚型之一,与抗原产生反应。IgG2a 在适应性免疫反应中发挥着至关重要的作用。IgG2A Fc 蛋白, Rat (HEK293) 是重组的 IgG2A Fc 蛋白,由 HEK293 表达,不带标签。IgG2A Fc 蛋白, Rat (HEK293) 全长 225 个氨基酸,分子量为 30-35 kDa。

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规格 价格 是否有货 数量
10 μg ¥680 In-stock
50 μg ¥1950 In-stock
100 μg   询价  

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描述

Ig gamma-2A chain C region (IgG2a) is one of the IgG antibodies subclasses and responses to an antigen. IgG2a plays a crucial role in the adaptive immune response[1][2]. IgG2A Fc Protein, Rat (HEK293) is the recombinant rat-derived IgG2A Fc protein, expressed by HEK293 , with tag free. The total length of IgG2A Fc Protein, Rat (HEK293) is 225 a.a., with molecular weight of 30-35 kDa.

研究背景

IgG2aIgG 抗体亚型之一。IgG2a 由 B 细胞产生并对抗原产生反应。IgG2a 单克隆抗体可以抑制小鼠肿瘤生长[1]IgG2a 通过调理作用/补体固定参与病原体防御和免疫效应器功能[2]

种属

Rat

表达系统

HEK293

标签

Tag Free

蛋白编号

P20760 (V98-K322)

基因 ID

679045

蛋白结构
N-term
IgG2A Fc (V98-K322)
Accession # P20760
C-term
同用名
Ig gamma-2 chain C region; IgG2A Fc
氨基酸序列

VPRECNPCGCTGSEVSSVFIFPPKTKDVLTITLTPKVTCVVVDISQNDPEVRFSWFIDDVEVHTAQTHAPEKQSNSTLRSVSELPIVHRDWLNGKTFKCKVNSGAFPAPIEKSISKPEGTPRGPQVYTMAPPKEEMTQSQVSITCMVKGFYPPDIYTEWKMNGQPQENYKNTPPTMDTDGSYFLYSKLNVKKETWQQGNTFTCSVLHEGLHNHHTEKSLSHSPGK

分子量

30-35 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, pH 8.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IgG2A Fc Protein, Rat (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IgG2A Fc Protein, Rat (HEK293)
目录号:
HY-P72604
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