1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-10
  5. IL-10 蛋白, Human (HEK293, His)

IL-10 蛋白, Human (HEK293, His)

目录号: HY-P73153
COA 产品使用指南

IL-10蛋白是一种主要的免疫调节细胞因子,具有深远的抗炎功能,可限制炎症引起的过度组织破坏。从机制上讲,IL10 与其异四聚体受体 IL10RA 和 IL10RB 结合,导致 JAK1 和 STAT2 介导的 STAT3 磷酸化,后者易位到细胞核并驱动抗炎介质的表达。IL-10 蛋白, Human (HEK293, His) 是重组的 IL-10 蛋白,由 HEK293 表达,带有 N-His 标签。IL-10 蛋白, Human (HEK293, His) 全长 160 个氨基酸,分子量约为 ~20.9 kDa。

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5 μg ¥1150 In-stock
10 μg ¥1850 In-stock
20 μg ¥2950 In-stock
50 μg ¥5600 In-stock
100 μg   询价  

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描述

IL-10 protein is a major immunoregulatory cytokine with profound anti-inflammatory functions that limits excessive tissue destruction caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptors IL10RA and IL10RB, leading to JAK1- and STAT2-mediated phosphorylation of STAT3, which translocates to the nucleus and drives the expression of anti-inflammatory mediators. IL-10 Protein, Human (HEK293, His) is the recombinant human-derived IL-10 protein, expressed by HEK293 , with N-His labeled tag. The total length of IL-10 Protein, Human (HEK293, His) is 160 a.a., with molecular weight of ~20.9 kDa.

研究背景

IL-10 蛋白是一种关键的免疫调节细胞因子,具有有效的抗炎作用,可防止炎症引起的过度组织损伤。它与由 IL10RA 和 IL10RB 组成的异四聚体受体结合,触发 JAK1 和 STAT2 介导的 STAT3 磷酸化。这导致 STAT3 易位到细胞核中,驱动抗炎介质的表达。IL-10 蛋白靶向巨噬细胞和单核细胞等抗原呈递细胞,抑制促炎细胞因子的释放。它还通过减少 MHC II 类和共刺激分子的表达来阻碍抗原呈递,从而抑制 T 细胞活化。此外,它还能重新编程代谢途径,包括 mTOR 信号传导,以控制巨噬细胞的炎症反应。该蛋白形成同二聚体并与 IL10RA 和 IL10RB 相互作用。

生物活性

1.Immobilized human IL10-His at 10 μg/mL (100 μl/well) can bind Cynomolgus IL10RA-Fc and the EC50 is 0.31-0.71 μg/mL.
2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells and the ED50 is typically 5-20 ng/mL.

种属

Human

表达系统

HEK293

标签

N-His

蛋白编号

P22301 (S19-N178)

基因 ID
蛋白结构
N-term
His
IL-10 (S19-N178)
Accession # P22301
C-term
同用名
Interleukin-10 receptor subunit beta; IL-10RB; CRF2-4; IL-10R2; CDw210b
分子量

Approximately 20.9 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

IL-10 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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