1. 重组蛋白
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors
  4. IL-17
  5. IL-17A
  6. IL-17A 蛋白, Mouse (HEK293, His-Avi)

IL-17A 蛋白, Mouse (HEK293, His-Avi)

目录号: HY-P78320
产品使用指南

IL-17A 是一种同型二聚体细胞因子,在宿主防御机制中对许多细菌和真菌病原体以及过敏和自身免疫反应起着关键作用。IL-17A 诱导抗菌肽、细胞因子、趋化因子和基质金属蛋白酶的产生。IL-17A 蛋白, Mouse (HEK293, His-Avi) 是一种重组小鼠 IL-17A 蛋白,在 HEK293 细胞中表达,C 末端带有 His 和Avi 标签,由 133 个氨基酸组成 (A26-A158)。

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50 μg   询价  
100 μg   询价  

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描述

IL-17A is a homodimeric cytokine and plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides, cytokines, chemokines, and matrix metalloproteinases[1][2]. IL-17A Protein, Mouse (HEK293, His-Avi) is a recombinant mouse IL-17A protein with His tag and Avi tag at the C-terminus and is expressed in HEK293 cells. It consists of 133 amino acids (A26-A158).

研究背景

Interleukin-17A (IL-17A), also known as CTLA-8, belongs to the IL-17 cytokine family. IL-17A is expressed in memory Th17 cells and is a product of memory CD4+ T cells. IL-17A is also produced by a wide variety of immune cells, including CD8+ T cells, γδT cells, natural killer T (NKT) cells, monocytes, and neutrophils[1][2][3].
The mouse IL-17A shares 63.23% amino acid sequence identity with human and 87.33% identity with rat.
IL-17A plays a critical role in host defense mechanisms against many bacterial and fungal pathogens as well as allergic and autoimmune responses. IL-17A induces the production of antimicrobial peptides (defensins and S100 proteins), cytokines (IL-6, G-CSF, and GM-CSF), chemokines (CXCL1, CXCL5, IL-8, CCL2, and CCL7), and matrix metalloproteinases (MMP1, MMP3, and MMP13). IL-17A is detrimental in viral infection through promoting neutrophilic inflammation. IL-17A is a homodimeric cytokine and shares similar biological activities with IL-17F. IL-17A binds to IL-17RA with high affinity, and IL-17RA is required for the biological activity of IL-17A. In tumorigenesis, IL-17A recruits myeloid derived suppressor cells (MDSCs) to dampen anti-tumor immunity. IL-17A also enhances tumor growth in vivo through the induction of IL-6[1][2].
IL-17A can be used for the research of autoimmune diseases, infection and cancer[1][4].

体内研究
(In Vivo)

Recombinant mouse interleukin 17 (rmIL-17) (0.1 or 0.9 μg/rat/day; nasally; 6 days) shows increased infiltration of inflammatory cells into the sciatic nerve, more severe demyelination, augmented proliferation of regional lymph node cells, and increased serum levels of tumor necrosis factor-α in chronic experimental autoimmune neuritis (EAN) induced Lewis rats[5].
rmIL-17A (625 ng/mouse; i.t.; once) dramatically synergizes TNF-alpha induced neutrophil accumulation in C57BL/6 mice[6].

生物活性

Immobilized Mouse IL-17A His at 1 μg/mL (100 μL/Well) on the plate. Dose response curve for Mouse IL-17RA hFc with the EC50 of 64.9 ng/mL determined by ELISA.

种属

Mouse

表达系统

HEK293

标签

C-Avi;C-His

蛋白编号

Q62386 (A26-A158)

基因 ID

16171  [NCBI]

中文名
重组小鼠白细胞介素-17A 蛋白 (HEK293, His-Avi)
同用名
CTLA-8; IL17; IL17A; IL-17CTLA-8; interleukin 17A
分子量

20-28 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5 % trehalose is added as protectant before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

IL-17A Protein, Mouse (HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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