IL-21 蛋白, Mouse (129a.a)

IL-21 Protein, Mouse (129a.a) is a regulatory cytokine thought to play a role in the transition between innate and adaptive immunity.

白介素 21 (IL-21) 是一种新型 I 型细胞因子，与 IL-2、IL-4 和 IL-15 显着同源。其受体复合物含有γc链，γc链也是IL-2、IL-4、IL-7、IL-9和IL-15受体的组成部分，因此它们的生物学功能可能存在重叠或相关性。IL-21能够共同刺激成熟T细胞、B细胞、NK细胞，并刺激NK细胞表面CD16表达，诱导先天免疫反应中的ADCC。它还可以增强细胞免疫的抗肿瘤作用，特别是通过增强NK和抗原特异性CTL细胞的活性。因此，IL-21 通过在荷瘤宿主中引发先天性和适应性抗肿瘤反应，成为恶性肿瘤免疫治疗的潜在有用治疗分子^[1]

1.The ED₅₀ is <1 ng/mL as measured by human ANBL-6 cells, corresponding to a specific activity of >1.0 × 10⁶ units/mg.
2.Measured by its binding ability in a functional ELISA. When Mouse IL-21 is coated at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-21R. The ED₅₀ for this effect is 17.34 ng/mL.

Mouse

E. coli

Tag Free

Q9ES17 (H18-S146)

60505  [NCBI]

HKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS

Approximately 15.1 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Tang W, et al. Expression, purification and identification of recombinant mouse interleukin 21 protein in E. coli. Cell Mol Immunol. 2006 Aug;3(4):311-5.  [Content Brief]