IL-22 蛋白, Mouse

IL-22 Protein, Mouse plays a prominent role in epithelial regeneration and dampening of chronic inflammatory responses by protecting intestinal stem cells from immune-mediated tissue damage.

白介素 22 (IL-22) 是细胞因子 IL-10 家族的成员，主要由先天淋巴细胞 (ILC) 和活化 T 细胞亚群表达，包括辅助性 T 1 (TH1) 细胞、TH17 细胞和 TH22 细胞。IL-22 可以被异二聚体受体复合物识别，该复合物由两个跨膜亚基组成：IL-22R1 和 IL-10R2。IL-22与其受体的结合激活JAK/STAT和MAPK信号通路，导致基因表达或抑制。由于 IL-10R2 是五种细胞因子（IL-10、IL-22、IL-26、IL-28 和 IL-29）共有的，并且在大多数细胞中广泛表达，因此 IL-22R1 的表达决定了细胞是IL-22的靶标。IL-22 在移植物抗宿主病 (GVHD) 方面具有相当大的治疗潜力，这是同种异体干细胞移植后常见且具有挑战性的并发症^[1]。

1.The ED₅₀ is <0.5 ng/mL as measured by COLO 205 cells.
2.Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 292.3 pg/mL, corresponding to a specific activity is 3.42×10⁶ U/mg.

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  Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED₅₀ for this effect is 292.3 pg/mL, corresponding to a specific activity is 3.42×10⁶ U/mg.

Mouse

E. coli

Tag Free

Q9JJY9 (L34-V179)

50929  [NCBI]

LPVNTRCKLEVSNFQQPYIVNRTFMLAKEASLADNNTDVRLIGEKLFRGVSAKDQCYLMKQVLNFTLEDVLLPQSDRFQPYMQEVVPFLTKLSNQLSSCHISGDDQNIQKNVRRLKETVKKLGESGEIKAIGELDLLFMSLRNACV

14-17 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized after extensive dialysis against PBS or 20 mM PB, 150 mM NaCl, pH 7.0 or 50 mM Tris-HCL, 300 mM NaCl, 10 mM Imidazole, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Lin Y, et al. Lactobacillus delivery of bioactive interleukin-22. Microb Cell Fact. 2017 Aug 23;16(1):148.  [Content Brief]