Integrin alpha M beta 2 蛋白, Human (HEK293, His)

Integrin α M β 2 (ITGAM/ITGB2) mediates adhesion of monocytes and granulocytes and promotes uptake of complement-coated particles and pathogens. As a receptor for iC3b and fibrinogen, it regulates neutrophil migration, contributes to CD177-PRTN3-mediated activation, and may influence phagocytosis-induced apoptosis. Integrin alpha M beta 2 Protein, Human (HEK293, His) is a recombinant protein dimer complex containing human-derived Integrin alpha M beta 2 protein, expressed by HEK293 , with C-His labeled tag. Integrin alpha M beta 2 Protein, Human (HEK293, His), has molecular weight of 140-160 kDa (ITGAM) & 90-100 kDa (ITGB2), respectively.

整合素 α M β 2，也称为 ITGAM/ITGB2，在介导单核细胞、巨噬细胞和粒细胞的粘附相互作用中发挥着至关重要的作用，促进补体包被颗粒和病原体的摄取。它充当第三补体成分的 iC3b 片段的受体，并可能识别 C3b 中的 RGD 肽。此外，该整合素复合物充当纤维蛋白原、因子 X 和 ICAM1 的受体，识别纤维蛋白原 γ 链内的特定肽。ITGAM/ITGB2 调节中性粒细胞迁移，对于 CD177-PRTN3 介导的 TNF 引发的中性粒细胞激活至关重要。它还可能有助于调节外渗中性粒细胞吞噬作用诱导的细胞凋亡，并在肥大细胞发育中发挥作用。此外，与 TYROBP/DAP12 结合，小胶质细胞需要它来控制超氧离子的产生，从而促进大脑发育过程中的神经元凋亡。与各种蛋白质（包括 JAM3 和 THBD）的相互作用凸显了整合素 α M β 2 在细胞过程中的多方面功能。此外，它与补体因子 H/CFH 的关联促进中性粒细胞与病原体的粘附，有助于病原体的清除。

Immobilized Human Integrin alpha M beta 2, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC₅₀ of 13.7 ng/mL determined by ELISA.

Human

HEK293

C-His

P11215-1 (F17-N1104)&P05107-1 (Q23-N700)

/&3689

140-160 kDa (ITGAM) & 90-100 kDa (ITGB2)

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.