JAML/AMICA 蛋白, Human (HEK293, His)

JAML is a leukocyte plasma membrane transmembrane protein that critically regulates cell migration and activation by interacting with CXADR on neighboring cells. This interaction activates γ-δ T cells and induces downstream signaling through PI3 kinase and MAP kinase. JAML/AMICA Protein, Human (HEK293, His) is the recombinant human-derived JAML/AMICA protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of JAML/AMICA Protein, Human (HEK293, His) is 256 a.a., with molecular weight of ~39 kDa.

JAML 是一种存在于白细胞质膜上的跨膜蛋白，通过与 CXADR（位于相邻上皮细胞和内皮细胞上的受体）结合，充当细胞迁移和激活的关键调节因子。这种相互作用对于激活 γ-δ T 细胞（参与组织稳态和修复的一个子集）至关重要，可诱导下游信号事件响应上皮 CXADR 结合。JAML 介导的信号传导在 PI3 激酶和 MAP 激酶的促进下，导致 T 细胞增殖以及细胞因子和生长因子的产生，从而促进组织修复。此外，JAML 通过与 CXADR 的粘附相互作用，在控制上皮和内皮组织内白细胞迁移方面发挥着关键作用。在其同型二聚体形式中，JAML 积极参与白细胞-内皮细胞粘附，突出其在协调免疫反应和组织修复过程中的多方面作用。此外，JAML 与整合素 ITGA4 和 ITGB1 的相互作用表明，通过调节 JAML 同二聚化来调节白细胞-内皮细胞粘附的机制。

Measured by the ability of the immobilized protein to support the adhesion of A549 Human non-small cell lung cancer cells. When 5×10⁴ cells/well are added to rhAMICA/Fc Chimera coated plates (10 µg/mL, 100 µL/well), approximately 87.96% will adhere after 1 hour at 37℃.

Human

HEK293

C-6*His

Q86YT9/NP_001091996.1 (L20-L275)

120425  [NCBI]

LNDLNVSPPELTVHVGDSALMGCVFQSTEDKCIFKIDWTLSPGEHAKDEYVLYYYSNLSVPIGRFQNRVHLMGDILCNDGSLLLQDVQEADQGTYICEIRLKGESQVFKKAVVLHVLPEEPKELMVHVGGLIQMGCVFQSTEVKHVTKVEWIFSGRRAKEEIVFRYYHKLRMSVEYSQSWGHFQNRVNLVGDIFRNDGSIMLQGVRESDGGNYTCSIHLGNLVFKKTIVLHVSPEEPRTLVTPAALRPLVLGGNQL

Approximately 39 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.