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  3. KRT18 蛋白, Human (HEK293, His)

KRT18 蛋白, Human (HEK293, His)

目录号: HY-P71668
COA 产品使用指南

KRT18 蛋白是具有 KRT8 的异四聚体,具有多种作用,包括摄取凝血酶-抗凝血酶复合物以及协助将突变的 CFTR 递送至质膜。它在 IL-6 介导的屏障保护中与 KRT8 合作,并与 PLEC 同工型 1C 相互作用。KRT18 蛋白, Human (HEK293, His) 是重组的 KRT18 蛋白,由 HEK293 表达,带有 N-10*His 标签。KRT18 蛋白, Human (HEK293, His) 全长 429 个氨基酸,分子量约为 ~50.8 kDa。

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描述

The KRT18 protein is a heterotetramer with KRT8 that has multiple roles, including uptake of thrombin-antithrombin complexes and assisting in the delivery of mutated CFTR to the plasma membrane. It cooperates with KRT8 in IL-6-mediated barrier protection and interacts with PLEC isoform 1C. KRT18 Protein, Human (HEK293, His) is the recombinant human-derived KRT18 protein, expressed by HEK293 , with N-10*His labeled tag. The total length of KRT18 Protein, Human (HEK293, His) is 429 a.a., with molecular weight of ~50.8 kDa.

研究背景

KRT18 蛋白在细胞过程中发挥多种作用,包括肝细胞摄取凝血酶-抗凝血酶复合物以及磷酸化时的细丝重组。它积极参与将突变的 CFTR 传递到质膜,并与 KRT8 合作实现白细胞介素 6 (IL-6) 介导的屏障保护。作为两种 I 型角蛋白和两种 II 型角蛋白的异四聚体,KRT18 与 KRT8 形成复合物,并在与 KRT8 形成异二聚体时与 PLEC 同工型 1C 相互作用。此外,KRT18 参与与凝血酶-抗凝血酶复合物的相互作用,并表现出与各种蛋白质的连接,例如 PNN、突变 CFTR、YWHAE、YWHAH、YWHAZ(磷酸化时)、DNAJB6、TCHP、TRADD、FAM83H、EPPK1、PKP1 和 PKP2。KRT18 的多方面相互作用和功能凸显了它对基本细胞活动的参与及其对控制细胞结构和信号传导的分子途径的贡献。

种属

Human

表达系统

HEK293

标签

N-10*His

蛋白编号

P05783 (S2-H430)

基因 ID
蛋白结构
N-term
10*His
KRT18 (S2-H430)
Accession # P05783
C-term
同用名
Cell proliferation inducing gene 46 protein; Cell proliferation inducing protein 46 ; Keratin D ; keratin, type I cytoskeletal 18; Keratin-18; Krt18
氨基酸序列

SFTTRSTFSTNYRSLGSVQAPSYGARPVSSAASVYAGAGGSGSRISVSRSTSFRGGMGSGGLATGIAGGLAGMGGIQNEKETMQSLNDRLASYLDRVRSLETENRRLESKIREHLEKKGPQVRDWSHYFKIIEDLRAQIFANTVDNARIVLQIDNARLAADDFRVKYETELAMRQSVENDIHGLRKVIDDTNITRLQLETEIEALKEELLFMKKNHEEEVKGLQAQIASSGLTVEVDAPKSQDLAKIMADIRAQYDELARKNREELDKYWSQQIEESTTVVTTQSAEVGAAETTLTELRRTVQSLEIDLDSMRNLKASLENSLREVEARYALQMEQLNGILLHLESELAQTRAEGQRQAQEYEALLNIKVKLEAEIATYRRLLEDGEDFNLGDALDSSNSMQTIQKTTTRRIVDGKVVSETNDTKVLRH

分子量

Approximately 50.8 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

KRT18 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
KRT18 Protein, Human (HEK293, His)
目录号:
HY-P71668
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