LAMP3/CD208 蛋白, Human (HEK293, His)

LAMP3/CD208 is a lysosomal glycoprotein that participates in the unfolded protein response (UPR) and contributes to protein degradation and cell survival, especially in the presence of proteasome dysfunction. It crucially promotes lysosome-autophagosome fusion and regulates autophagy. LAMP3/CD208 Protein, Human (HEK293, His) is the recombinant human-derived LAMP3/CD208 protein, expressed by HEK293 , with C-His labeled tag. The total length of LAMP3/CD208 Protein, Human (HEK293, His) is 354 a.a., with molecular weight of 70-100 kDa.

LAMP3/CD208 是一种溶酶体膜糖蛋白，参与未折叠蛋白反应 (UPR)，有助于蛋白质降解和细胞存活，特别是在蛋白酶体功能障碍期间。此外，它在溶酶体和自噬体之间的融合过程中起着至关重要的作用，从而调节自噬。LAMP3/CD208 已被确定为通过激活 PI3K/Akt 途径而促进肝细胞脂肪生成的启动子。此外，它还与树突细胞功能和适应性免疫有关。在微生物感染的背景下，LAMP3/CD208在甲型流感病毒复制的进入后步骤中发挥积极作用，影响病毒脱壳、细胞质运输或病毒成分的核输入等过程，并有助于流感病毒的核积累病毒感染早期阶段的核蛋白/NP。

Measured by its ability to enhance MDA-MB-231 cells migration. The ED₅₀ for this effect is 0.401μg/mL, corresponding to a specific activity is 2.494×10³ U/mg.

Human

HEK293

C-6*His

Q9UQV4/NP_055213.2 (K28-T381)

27074  [NCBI]

KAFPETRDYSQPTAAATVQDIKKPVQQPAKQAPHQTLAARFMDGHITFQTAATVKIPTTTPATTKNTATTSPITYTLVTTQATPNNSHTAPPVTEVTVGPSLAPYSLPPTITPPAHTTGTSSSTVSHTTGNTTQPSNQTTLPATLSIALHKSTTGQKPVQPTHAPGTTAAAHNTTRTAAPASTVPGPTLAPQPSSVKTGIYQVLNGSRLCIKAEMGIQLIVQDKESVFSPRRYFNIDPNATQASGNCGTRKSNLLLNFQGGFVNLTFTKDEESYYISEVGAYLTVSDPETIYQGIKHAVVMFQTAVGHSFKCVSEQSLQLSAHLQVKTTDVQLQAFDFEDDHFGNVDECSSDYT

Approximately 70-100 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.