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MICB 蛋白, Human (HEK293, His)

目录号: HY-P73294
COA 产品使用指南

MICB 蛋白是 NKG2D 受体的高度糖基化配体,可触发免疫细胞的溶细胞反应。它类似于 MHC I 类分子,但缺乏与 β-2-微球蛋白或肽结合的关联。选择性剪接导致多种转录变体。MICB 广泛表达,特别是在淋巴结和骨髓中,突出了其在不同生理环境中的免疫反应作用。MICB 蛋白, Human (HEK293, His) 是重组的 MICB 蛋白,由 HEK293 表达,带有 C-His, C-6*His 标签。MICB 蛋白, Human (HEK293, His) 全长 276 个氨基酸,分子量约为 ~45 kDa。

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5 μg ¥400 In-stock
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1 mg ¥20000 In-stock
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描述

MICB Protein, a heavily glycosylated ligand for NKG2D receptor, triggers cytolytic response in immune cells. It resembles MHC class I molecules but lacks association with beta-2-microglobulin or peptide binding. Alternative splicing leads to multiple transcript variants. MICB is widely expressed, especially in lymph nodes and bone marrow, highlighting its immune response role in diverse physiological contexts. MICB Protein, Human (HEK293, His) is the recombinant human-derived MICB protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of MICB Protein, Human (HEK293, His) is 276 a.a., with molecular weight of ~45 kDa.

研究背景

MICB 编码一种高度糖基化的蛋白质,作为 NKG2D II 型受体的配体。结合后,该配体会触发各种免疫细胞的溶细胞反应,包括自然杀伤 (NK) 细胞、CD8 Alphata T 细胞和表达该受体的 gammadelta T 细胞。值得注意的是,MICB 是应激诱导的,并且与 MHC I 类分子具有相似性,尽管它不与 β-2-微球蛋白结合或结合肽。选择性剪接产生多个转录变体,增加了其功能库的复杂性。MICB 的表达范围广泛,在淋巴结、骨髓和各种其他组织中显着存在,强调了其在不同生理环境下的免疫反应中的作用。

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

NP_005922.2 (A23-G298)

基因 ID
蛋白结构
N-term
MICB (A23-G298)
Accession # NP_005922.2
6*His
C-term
同用名
MHC class I polypeptide-related sequence B; MIC-B; PERB11.2
氨基酸序列

AEPHSLRYNLMVLSQDGSVQSGFLAEGHLDGQPFLRYDRQKRRAKPQGQWAENVLGAKTWDTETEDLTENGQDLRRTLTHIKDQKGGLHSLQEIRVCEIHEDSSTRGSRHFYYDGELFLSQNLETQESTVPQSSRAQTLAMNVTNFWKEDAMKTKTHYRAMQADCLQKLQRYLKSGVAIRRTVPPMVNVTCSEVSEGNITVTCRASSFYPRNITLTWRQDGVSLSHNTQQWGDVLPDGNGTYQTWVATRIRQGEEQRFTCYMEHSGNHGTHPVPSG

分子量

Approximately 45 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

MICB Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
MICB Protein, Human (HEK293, His)
目录号:
HY-P73294
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