MMP-2 蛋白, Human (HEK293)

MMP-2 protein is a multifunctional metalloproteinase that actively participates in physiological processes such as vascular remodeling, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. In addition to degrading extracellular matrix proteins, it also acts on non-matrix proteins to promote vasoconstriction. MMP-2 Protein, Human (HEK293) is the recombinant human-derived MMP-2 protein, expressed by HEK293 , with tag free. The total length of MMP-2 Protein, Human (HEK293) is 631 a.a., with molecular weight of ~72 kDa.

MMP-2 蛋白是一种普遍存在的金属蛋白酶，积极参与一系列生理过程，包括脉管系统重塑、血管生成、组织修复、肿瘤侵袭、炎症和动脉粥样硬化斑块破裂。除了降解细胞外基质蛋白的作用外，该蛋白还通过作用于非基质蛋白（例如大内皮 1 和 β 型 CGRP）而表现出多功能性，从而促进血管收缩。此外，它在 Gly-|-Leu 键处裂解 KISS，并且似乎在心肌细胞死亡途径中发挥作用。通过调节 GSK3beta 的活性并在体外裂解 GSK3beta，它有助于心肌氧化应激。MMP-2 与 MMP14 相关，参与纤维血管组织的形成。值得注意的是，MMP-2 的 C 端非催化片段（称为 PEX）具有抗血管生成和抗肿瘤特性，可抑制细胞迁移和与 FGF2 和玻连蛋白的粘附。此外，它还充当血管表面整合素 α-v/β3 的配体。

1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 and the specific activity is > 1,000 pmoles/min/µg. (Activation description: The proenzyme needs to be activated by APMA for an activated form).
2. Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 at 2 μg/mL (100 μl/well) can bind Human TIMP2 hFc and the EC₅₀ is 6.0-30.0 ng/mL.

• 
  Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is 11011 pmol/min/µg, as measured under the described conditions.

Human

HEK293

Tag Free

P08253/NP_004521.1 (A30-C660)

4313  [NCBI]

APSPIIKFPGDVAPKTDKELAVQYLNTFYGCPKESCNLFVLKDTLKKMQKFFGLPQTGDLDQNTIETMRKPRCGNPDVANYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESCTSAGRSDGKMWCATTANYDDDRKWGFCPDQGYSLFLVAAHEFGHAMGLEHSQDPGALMAPIYTYTKNFRLSQDDIKGIQELYGASPDIDLGTGPTPTLGPVTPEICKQDIVFDGIAQIRGEIFFFKDRFIWRTVTPRDKPMGPLLVATFWPELPEKIDAVYEAPQEEKAVFFAGNEYWIYSASTLERGYPKPLTSLGLPPDVQRVDAAFNWSKNKKTYIFAGDKFWRYNEVKKKMDPGFPKLIADAWNAIPDNLDAVVDLQGGGHSYFFKGAYYLKLENQSLKSVKFGSIKSDWLGC

Approximately 72 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 0.05% Brij-35, 150 mM NaCl, 5 mM CaCl₂, 50 mM Tris, pH 7.5 or 50 mM Tris, 150 mM NaCl, 5 mM CaCl2, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.