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  4. Outer membrane porin C/OmpC 蛋白, E.coli (Myc, His-SUMO)

Outer membrane porin C/OmpC 蛋白, E.coli (Myc, His-SUMO)

目录号: HY-P71610
产品使用指南

外膜孔蛋白 C/OmpC 蛋白促进物质穿过细菌外膜的运输，形成用于小分子被动扩散的同源三聚体孔，这对于维持细胞包膜通透性至关重要。Outer membrane porin C/OmpC 蛋白, E.coli (Myc, His-SUMO) 是重组的 外膜孔蛋白 C/OmpC 蛋白，由 E. coli 表达，带有 N-His, C-Myc, N-SUMO 标签。Outer membrane porin C/OmpC 蛋白, E.coli (Myc, His-SUMO) 全长 346 个氨基酸，分子量约为 ~58.4 kDa。

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描述

Outer membrane porin C/OmpC Protein facilitates substance transport across the bacterial outer membrane, forming homotrimeric pores for passive diffusion of small molecules, crucial for maintaining cell envelope permeability. Outer membrane porin C/OmpC Protein, E.coli (Myc, His-SUMO) is the recombinant E. coli-derived Outer membrane porin C/OmpC protein, expressed by E. coli , with N-His, C-Myc, N-SUMO labeled tag. The total length of Outer membrane porin C/OmpC Protein, E.coli (Myc, His-SUMO) is 346 a.a., with molecular weight of ~58.4 kDa.
研究背景

外膜孔蛋白 C/OmpC 蛋白通过形成孔来发挥作用，使小分子能够被动扩散穿过外膜。该蛋白质以同源三聚体形式存在，在促进物质穿过细菌外膜的运输方面发挥着至关重要的作用，有助于细胞膜的通透性。
种属

E.coli
表达系统

E. coli
标签

N-His;C-Myc;N-SUMO
蛋白编号

Q8XE41 (22A-367F)
基因 ID

916811  [NCBI]
蛋白结构
N-term
10*His-SUMO
OmpC (22A-367F)
Accession # Q8XE41
C-term
同用名
ompC; Z3473; ECs3104; Outer membrane porin C; Outer membrane protein 1B; Outer membrane protein C; Porin OmpC
氨基酸序列

AEVYNKDGNKLDLYGKVDGLHYFSDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLKFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGLVDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSKRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF

分子量

Approximately 58.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Outer membrane porin C/OmpC Protein, E.coli (Myc, His-SUMO) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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