1. 重组蛋白
  2. Cytokines and Growth Factors
  3. EGF Superfamily
  4. Betacellulin
  5. Probetacellulin 蛋白, Human (HEK293)

Probetacellulin 蛋白, Human (HEK293)

目录号: HY-P7328
COA 产品使用指南

Probetacellulin 蛋白, Human (HEK293)是 HEK293 细胞中产生的一种糖蛋白,能够通过提高来自导管和胰腺腺泡细胞的 β 细胞的分化和再生来提高葡萄糖耐受性。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥4200 In-stock
100 μg   询价  
250 μg   询价  

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描述

Probetacellulin Protein, Human (HEK293) is a glycoprotein produced in HEK293 cells, improves glucose tolerance by promoting β-cell differentiation and regeneration from ductal and/or acinar cells.

研究背景

Betacellulin (BTC), a member of the epidermal growth factor family, is expressed predominantly in the human pancreas and induces the differentiation of a pancreatic acinar cell line (AR42J) into insulin-secreting cells. Betacellulin binds and activates the EGF receptor (EGFR/erbB-1) and erbB-4, and it induces tyrosine phosphorylation of erbB-2, which couples with the EGFR or erbB-4[1].

生物活性

The ED50 is <4 pg/mL as measured by 3T3 cells.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P35070 (D32-Y111)

基因 ID

685  [NCBI]

同用名
rHuBetacellulin; BTC
氨基酸序列

DGNSTRSPETNGLLCGDPEENCAATTTQSKRKGHFSRCPKQYKHYCIKGRCRFVVAEQTPSCVCDEGYIGARCERVDLFY

分子量

15-18 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

Probetacellulin Protein, Human (HEK293) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Probetacellulin Protein, Human (HEK293)
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HY-P7328
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