1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. RET 蛋白, Human (HEK293, His)

RET 蛋白, Human (HEK293, His)

目录号: HY-P73679
COA 产品使用指南

RET 蛋白是一种受体酪氨酸蛋白激酶,与胶质细胞源性神经营养因子家族配体结合后,在细胞增殖、神经元导航和分化中发挥关键作用。它调节细胞死亡和存活之间的微妙平衡,影响位置信息,并且对于肠器官发生、肠神经系统发育、肾器官发生和派尔氏集结形成至关重要。RET 蛋白, Human (HEK293, His) 是重组的 RET 蛋白,由 HEK293 表达,带有 N-His 标签。RET 蛋白, Human (HEK293, His) 全长 607 个氨基酸,分子量为 110-120 kDa。

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50 μg ¥4200 In-stock
100 μg   询价  

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描述

RET protein is a receptor tyrosine protein kinase that plays a key role in cell proliferation, neuronal navigation, and differentiation upon binding to glial cell-derived neurotrophic factor family ligands. It regulates the delicate balance between cell death and survival, influences positional information, and is critical for intestinal organogenesis, enteric nervous system development, renal organogenesis, and Peyer's patch formation. RET Protein, Human (HEK293, His) is the recombinant human-derived RET protein, expressed by HEK293 , with N-His labeled tag. The total length of RET Protein, Human (HEK293, His) is 607 a.a., with molecular weight of 110-120 kDa.

研究背景

RET 蛋白是一种受体酪氨酸蛋白激酶,在与神经胶质细胞源性神经营养因子家族配体结合后,在细胞增殖、神经元导航、迁移和分化等多种细胞过程中发挥着至关重要的作用。它磷酸化 PTK2/FAK1 并调节细胞死亡和存活以及位置信息之间的微妙平衡。RET 对于肠器官发生过程中的分子协调至关重要,它参与肠神经系统的发育、肾器官发生,并促进肠道相关淋巴组织中派尔氏斑样结构的形成。此外,RET 通过交感神经元中的半胱天冬酶裂解介导调节细胞粘附,并以整合素依赖性方式促进细胞迁移。作为依赖性受体,它在生长激素细胞中缺乏配体 GDNF 的情况下触发细胞凋亡,但在其存在的情况下促进存活并下调生长激素的产生。RET 是多种疾病的关键介质,特别是以异常整合素调节的细胞迁移为特征的神经内分泌癌。此外,它通过与 GFRAL 相互作用介导 GDF15 诱导的脑干细胞信号传导,从而抑制食物摄入并激活 MAPK 和 AKT 信号通路。值得注意的是,与同一复合物中的异构体 2 相比,与 GFRAL 复合的异构体 1 诱导 MAPK 信号通路更高的激活。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

N-His

蛋白编号

P07949 (L29-R635)

基因 ID
蛋白结构
N-term
His
RET (L29-R635)
Accession # P07949
C-term
同用名
Proto-oncogene tyrosine-protein kinase receptor Ret; RET; CDHF12; CDHR16
氨基酸序列

LYFSRDAYWEKLYVDQAAGTPLLYVHALRDAPEEVPSFRLGQHLYGTYRTRLHENNWICIQEDTGLLYLNRSLDHSSWEKLSVRNRGFPLLTVYLKVFLSPTSLREGECQWPGCARVYFSFFNTSFPACSSLKPRELCFPETRPSFRIRENRPPGTFHQFRLLPVQFLCPNISVAYRLLEGEGLPFRCAPDSLEVSTRWALDREQREKYELVAVCTVHAGAREEVVMVPFPVTVYDEDDSAPTFPAGVDTASAVVEFKRKEDTVVATLRVFDADVVPASGELVRRYTSTLLPGDTWAQQTFRVEHWPNETSVQANGSFVRATVHDYRLVLNRNLSISENRTMQLAVLVNDSDFQGPGAGVLLLHFNVSVLPVSLHLPSTYSLSVSRRARRFAQIGKVCVENCQAFSGINVQYKLHSSGANCSTLGVVTSAEDTSGILFVNDTKALRRPKCAELHYMVVATDQQTSRQAQAQLLVTVEGSYVAEEAGCPLSCAVSKRRLECEECGGLGSPTGRCEWRQGDGKGITRNFSTCSPSTKTCPDGHCDVVETQDINICPQDCLRGSIVGGHEPGEPRGIKAGYGTCNCFPEEEKCFCEPEDIQDPLCDELCR

分子量

110-120 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RET Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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RET Protein, Human (HEK293, His)
目录号:
HY-P73679
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