RNF43 蛋白, Human (HEK293, His)

RNF43 protein is encoded by the RNF43 gene and is a RING-type E3 ubiquitin ligase. It negatively regulates Wnt signaling by increasing ubiquitination of Frizzled receptors, resulting in reduced surface levels of these receptors. RNF43 Protein, Human (HEK293, His) is the recombinant human-derived RNF43 protein, expressed by HEK293 , with C-His labeled tag. The total length of RNF43 Protein, Human (HEK293, His) is 174 a.a., with molecular weight of ~25-38 kDa.

RNF43 基因编码 RING 型 E3 泛素连接酶，预计包含跨膜结构域、蛋白酶相关结构域、胞外结构域和细胞质 RING 结构域。该蛋白参与 Wnt 信号传导的负调节，通过增加卷曲受体的泛素化并诱导其亚细胞分布的改变来发挥其影响，最终导致这些受体的表面水平降低。值得注意的是，该基因的突变已在多种肿瘤细胞中被发现，包括与结直肠癌和子宫内膜癌相关的细胞。该基因表现出广泛的表达，在十二指肠 (RPKM 7.1)、结肠 (RPKM 6.1) 和 24 个其他组织中观察到显着水平。RNF43 的多方面作用凸显了其在调节关键细胞途径中的重要性及其在肿瘤发生中的潜在影响。

Human R-Spondin 3, His Tag immobilized on CM5 Chip can bind Human RNF43, His Tag with an affinity constant of 0.255 μM as determined in SPR assay (Biacore T200).

Human

HEK293

C-His

NP_060233.3 (G24-Y197)

54894  [NCBI]

GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY

Approximately 25-38 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE. The protein migrates as a 25-30 kDa band under reducing SDS-PAGE due to glycosylation.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.