S100A9 蛋白, Mouse (His)

S100A9 Protein is a calcium- and zinc-binding protein which belongs to the S100 family that primarily expresses in neutrophils and monocytes. S100A9 can induce neutrophil chemotaxis, adhesion, can increase the bactericidal activity of neutrophils by promoting phagocytosis via activation of SYK, PI3K/AKT, and ERK1/2. S100A9 has pro-inflammatory, antimicrobial, oxidant-scavenging and apoptosis-inducing activities which plays an important role in the regulation of inflammatory processes and immune response. S100A9 Protein, Mouse (His) is the recombinant mouse-derived S100A9 protein, expressed by E. coli , with N-His labeled tag. The total length of S100A9 Protein, Mouse (His) is 112 a.a., with molecular weight of 16-20 kDa.

S100A9 是一种钙和锌结合蛋白，属于 S100 家族，主要在中性粒细胞和单核细胞中表达。S100A9 可诱导中性粒细胞趋化、粘附，可通过激活 SYK、PI3K/AKT 和 ERK1/2，促进吞噬作用来增加中性粒细胞的杀菌活性，并可通过 MAPK 依赖性机制诱导中性粒细胞脱颗粒。S100A9 促进白细胞花生四烯酸转运和代谢、吞噬细胞迁移过程中微管蛋白依赖性细胞骨架调节以及中性粒细胞 NADPH 氧化酶的激活。S100A9 具有促炎、抗菌、清除氧化剂和诱导细胞凋亡的活性。S100A9 充当警报素或危险相关分子模式 (DAMP) 分子，并通过与模式识别受体结合来刺激先天免疫细胞 (TLR4) 和晚期糖基化终产物受体 (AGER)。S100A9 与 TLR4 结合，AGER 激活 MAP 激酶和 NF-kappa-B 信号通路，导致促炎级联放大。S100A9 对细菌和真菌具有抗菌活性，并可能通过对微生物生长至关重要的 Zn2+ 螯合发挥其抗菌活性。S100A9可以通过抗凋亡作用调节中性粒细胞数量和细胞凋亡；通过 ITGAM/ITGB 和 TLR4 以及涉及 MEK-ERK 的信号传导机制调节细胞存活。S100A9 在炎症过程和免疫反应的调节中发挥重要作用^{[1][2][3][4][5][6][7]}。

Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells .The ED₅₀ for this effect is 0.4264-0.8299 μg/mL, corresponding to a specific activity is 1.20× 10³-2.345 × 10³ units/mg.

• 
  Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.8299 μg/mL, corresponding to a specific activity is 1.20× 10³ units/mg determined by qRT-PCR.
• 
  Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.4264 μg/mL, corresponding to a specific activity is 2.345 × 10³ units/mg determined by ELISA.

Mouse

E. coli

N-His

P31725 (A2-K113)

20202  [NCBI]

ANKAPSQMERSITTIIDTFHQYSRKEGHPDTLSKKEFRQMVEAQLATFMKKEKRNEALINDIMEDLDTNQDNQLSFEECMMLMAKLIFACHEKLHENNPRGHGHSHGKGCGK

16-20 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of sterile 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80 or PBS, 1 mM DTT, 20% glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.