SCF 蛋白, Rat (His)

Stem cell factor (SCF), as a key ligand of KIT, regulates cell survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving phosphorylation events, key kinases (AKT1, RAS, RAF1, MAPK1/ERK2, MAPK3/ERK1), STAT family members, and PLCG1. SCF Protein, Rat (His) is the recombinant rat-derived SCF protein, expressed by E. coli , with C-6*His labeled tag. The total length of SCF Protein, Rat (His) is 164 a.a., with molecular weight of ~17 kDa.

干细胞因子（SCF）作为受体型蛋白酪氨酸激酶KIT的关键配体，在细胞存活、增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和黑素生成中发挥不可或缺的调节功能。与 KIT 结合后，SCF 激活多种信号传导途径，触发磷酸化事件，从而激活 AKT1 等关键激酶和 MAP 激酶级联的组件，包括 RAS、RAF1 和 MAPK1/ERK2 或 MAPK3/ERK1。此外，SCF 驱动的信号传导涉及 STAT 家族成员（STAT1、STAT3 和 STAT5）和 PLCG1 的激活，最终产生关键的细胞信号传导分子。SCF 与其他细胞因子（可能是白细胞介素）协同作用，形成同二聚体并与 KIT 非共价连接，从而形成异四聚体，介导 KIT 二聚化并随后通过自磷酸化激活。这个复杂的网络强调了 SCF 在协调不同细胞过程和发育途径方面的多方面作用。

Rat

E. coli

C-6*His

P21581 (Q26-A189)

60427  [NCBI]

QEICRNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVTHLSVSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVACMEENAPKNVKESLKKPETRNFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA

Approximately 17 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.