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SHH 蛋白, Mouse

目录号: HY-P7290
COA 产品使用指南

SHH 蛋白, Mouse是一种能够协调多种小脑发育成熟过程的形态发生因子。

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规格 价格 是否有货 数量
Free Sample   Apply now  
2 μg ¥350 In-stock
10 μg ¥1000 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

SHH Protein, Mouse is a morphogenic factor that actively orchestrates many aspects of cerebellar development and maturation.

研究背景

声波刺猬 (Shh) 是一种形态发生因子,可积极协调小脑发育和成熟的许多方面[1]。音速刺猬 (Shh) 在产后骨骼肌再生中起着至关重要的作用。Sonic Hedgehog (Shh) 是一种重要的形态发生素,在胚胎发生过程中调节上皮-间质相互作用。在成人中,Shh 通路已被证明在骨骼肌和心肌缺血后上调,这表明胚胎 Shh 通路可以被募集[2]

生物活性

The ED50 is <1 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >1.0 × 103 units/mg.

  • Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.05569 µg/mL, corresponding to a specific activity is 1.80×104 U/mg units/mg.
种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

Q62226 (C25-G198, C25I-V-I)

基因 ID

20423  [NCBI]

蛋白结构
N-term
SHH (C25-G198, C25I-V-I)
Accession # Q62226
C-term
中文名
重组鼠SHH蛋白
同用名
rMuShh; HHG-1; ShhNC
氨基酸序列

IVIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

分子量

Approximately 19.8 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

SHH Protein, Mouse 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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