SLAMF6 蛋白, Mouse (HEK293, His)

SLAMF6 protein is a self-ligand receptor in the SLAM family, which complexly regulates the activation and differentiation of immune cells through homotypic or heterotypic interactions, affecting innate and adaptive immune responses. It relies on cytoplasmic adapters such as SH2D1A/SAP and/or SH2D1B/EAT-2 for activity control. SLAMF6 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SLAMF6 protein, expressed by HEK293 , with C-His labeled tag. The total length of SLAMF6 Protein, Mouse (HEK293, His) is 209 a.a., with molecular weight of ~28-50 kDa.

SLAMF6 蛋白作为信号淋巴细胞激活分子 (SLAM) 家族中的自配体受体发挥作用，参与同型或异型细胞间相互作用，调节各种免疫细胞的激活和分化，有助于两者的调节和协调。先天性和适应性免疫反应。SLAMF6 的活性受到小细胞质接头蛋白（包括 SH2D1A/SAP 和/或 SH2D1B/EAT-2）的存在或不存在的复杂控制。具体来说，在表达高表面密度天然细胞毒性受体的自然杀伤 (NK) 细胞中，SLAMF6 触发细胞溶解活性，并暗示涉及 VAV1 磷酸化的正信号传导。此外，SLAMF6 与 SLAMF1 一起控制胸腺自然杀伤 T (NKT) 细胞谱系的正选择与随后的扩增和分化之间的过渡。该蛋白还促进 T 细胞分化为辅助 T 细胞 Th17 表型，导致 IL-17 分泌增加，其共刺激活性需要 SH2D1A。此外，SLAMF6 与 SLAMF1 和 CD84/SLAMF5 结合，可能充当体液免疫反应的负调节因子。在缺乏 SH2D1A/SAP 的情况下，SLAMF6 向 CD4(+) T 细胞和 NKT 细胞传递负信号，通过抑制 T 细胞：B 细胞粘附来负向调节生发中心形成，可能涉及增加与 PTPN6/SHP-1 的关联通过 ITSM。然而，相互矛盾的报道表明其在介导 T 细胞粘附、参与稳定的 T 细胞：B 细胞相互作用以及维持生发中心 B 细胞耐受性以预防自身免疫方面发挥作用。此外，SLAMF6 参与自身免疫的调节，而异构体 3 可能充当致病性 T 细胞增殖的抑制剂。该蛋白形成同二聚体，并在磷酸化后与 PTN6、PTN11 和 SH2D1A/SAP 相互作用。

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse SLAMF6 at 2 μg/mL (100 μL/well) can bind biotinylated Human SH2D1A. The ED₅₀ for this effect is 24.13 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse SLAMF6 at 2 μg/mL (100 μL/well) can bind biotinylated Human SH2D1A .The ED₅₀ for this effect is 24.13 ng/mL.

Mouse

HEK293

C-His

Q9ET39 (E31-N239)

30925  [NCBI]

EVSQSSSDPQLMNGVLGESAVLPLKLPAGKIANIIIWNYEWEASQVTALVINLSNPESPQIMNTDVKKRLNITQSYSLQISNLTMADTGSYTAQITTKDSEVITFKYILRVFERLGNLETTNYTLLLENGTCQIHLACVLKNQSQTVSVEWQATGNISLGGPNVTIFWDPRNSGDQTYVCRAKNAVSNLSVSVSTQSLCKGVLTNPPWN

The protein migrates as an approximately 28-50 kDa band under reducing SDS-PAGE due to glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.