SMARCA4 蛋白, Human (P.pastoris, His)

The SMARCA4 protein is a multifaceted regulator of chromatin remodeling that dynamically alters DNA nucleosome topology through ATP-dependent enzymatic activity. As part of the SWI/SNF complex and the CREST-BRG1 complex, it regulates transcriptional processes, coordinating the release and activating recruitment of calcium-dependent repressor complexes. SMARCA4 Protein, Human (P.pastoris, His) is the recombinant human-derived SMARCA4 protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of SMARCA4 Protein, Human (P.pastoris, His) is 547 a.a., with molecular weight of ~64.8 kDa.

SMARCA4 蛋白是一种多方面的调节因子，通过染色质重塑参与转录激活和抑制，从而导致 DNA-核小体拓扑结构的改变。作为 SWI/SNF 染色质重塑复合物的组成部分，它发挥 ATP 依赖性酶活性，通过影响核小体内 DNA-组蛋白接触来动态修改染色质结构。在 CREST-BRG1 复合物的背景下，SMARCA4 参与协调钙依赖性阻遏物复合物的释放和激活物复合物的募集，从而调节转录过程。特别是在神经发育中，SMARCA4 对于从增殖的神经干/祖细胞向有丝分裂后神经元的转变至关重要，充当 ZEB1 的辅阻遏物并促进 E-钙粘蛋白转录。此外，它与各种蛋白质进行多种相互作用，有助于其参与基本的细胞功能，包括生长调节、染色质重塑和转录控制。SMARCA4 在这些过程中的复杂作用凸显了它在细胞分化、染色质动力学和基因表达调控中的重要性。

Human

P. pastoris

N-6*His

P51532 (E700-F1246)

6597  [NCBI]

EVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAF

Approximately 64.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.