2. 重组蛋白
  3. Spondin-2/SPON2 蛋白, Human (Q9BUD6, HEK293, His)

Spondin-2/SPON2 蛋白, Human (Q9BUD6, HEK293, His)

目录号: HY-P70142A
产品使用指南

Spondin-2/SPON2蛋白作为细胞粘附分子，在促进海马胚胎神经元粘附和生长中发挥关键作用。除了在神经发育中发挥作用外，SPON2 还可以作为细菌及其成分的直接结合剂，作为调理素促进巨噬细胞对细菌的吞噬作用。Spondin-2/SPON2 蛋白, Human (Q9BUD6, HEK293, His) 是重组的 Spondin-2/SPON2 蛋白，由 HEK293 表达，带有His 标签。Spondin-2/SPON2 蛋白, Human (Q9BUD6, HEK293, His) 全长 305 个氨基酸，分子量为 38-42 kDa。

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描述

Spondin-2/SPON2 protein, as a cell adhesion molecule, plays a key role in promoting the adhesion and growth of hippocampal embryonic neurons. In addition to its role in neurodevelopment, SPON2 acts as a direct binder of bacteria and their components, functioning as an opsonin to promote phagocytosis of bacteria by macrophages. Spondin-2/SPON2 Protein, Human (Q9BUD6, HEK293, His) is the recombinant human-derived Spondin-2/SPON2 protein, expressed by HEK293 , with His labeled tag. The total length of Spondin-2/SPON2 Protein, Human (Q9BUD6, HEK293, His) is 305 a.a., with molecular weight of 38-42 kDa.
研究背景

Spondin-2，也称为 SPON2，是一种多功能细胞粘附蛋白，对于海马胚胎神经元粘附和生长至关重要。其功能多样性延伸到结合细菌及其成分，作为促进巨噬细胞吞噬细菌的调理素。SPON2 对于启动先天免疫反应至关重要，它在微生物病原体的细胞外基质 (ECM) 中充当独特的模式识别分子，特异性结合细菌脂多糖 (LPS)。SPON2 作为单体存在，与整合素相互作用，进一步强调了其在介导细胞-基质相互作用中的作用（参考：提供的信息）。
种属

Human
表达系统

HEK293
标签

His
蛋白编号

Q9BUD6 (Q27-V331)
基因 ID

10417
蛋白结构
N-term
SPON2 (Q27-V331)
Accession # Q9BUD6
10*His
C-term
同用名
rHuSpondin-2/SPON2, His; Spondin-2; Differentially expressed in cancerous and non-cancerous lung cells 1; DIL-1; Mindin; SPON2
氨基酸序列

QPLGGESICSARALAKYSITFTGKWSQTAFPKQYPLFRPPAQWSSLLGAAHSSDYSMWRKNQYVSNGLRDFAERGEAWALMKEIEAAGEALQSVHEVFSAPAVPSGTGQTSAELEVQRRHSLVSFVVRIVPSPDWFVGVDSLDLCDGDRWREQAALDLYPYDAGTDSGFTFSSPNFATIPQDTVTEITSSSPSHPANSFYYPRLKALPPIARVTLVRLRQSPRAFIPPAPVLPSRDNEIVDSASVPETPLDCEVSLWSSWGLCGGHCGRLGTKSRTRYVRVQPANNGSPCPELEEEAECVPDNCV

分子量

38-42 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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