Tenascin/Tnc 蛋白, Mouse (His-Myc)

Tenascin/Tnc proteins guide neuronal and axonal migration and contribute to development, synaptic plasticity, and neuronal regeneration. Tenascin/Tnc protein, Mouse (His-Myc) is the recombinant mouse-derived Tenascin/Tnc protein, expressed by HEK293 , with N-10*His, C-Myc labeled tag. The total length of Tenascin/Tnc protein, Mouse (His-Myc) is 216 a.a., with molecular weight of 27-31 kDa.

Tenascin/Tnc 蛋白是一种细胞外基质蛋白，在发育过程中引导神经元和轴突迁移以及促进突触可塑性和神经元再生方面发挥着关键作用。它不仅促进培养神经元中的神经突生长，而且可能在支持上皮肿瘤的生长中发挥作用，强调了其多样化的功能意义。作为整合素 ITGA8:ITGB1、ITGA9:ITGB1、ITGAV:ITGB3 和 ITGAV:ITGB6 的配体，Tenascin/Tnc 建立了细胞通讯和信号转导所必需的分子相互作用。在肿瘤中，它通过促进内皮细胞的伸长、迁移和萌芽来刺激血管生成。在结构上，Tenascin/Tnc 以同源六聚体形式存在，在三卷曲螺旋区域可能形成同源三聚体，并通过两端的二硫环进一步稳定。与 CSPG4 的相互作用表明它参与不同生物环境中复杂的细胞和分子过程。

Measured by the ability of the immobilized protein to block Fibronectin-mediated adhesion of NIH-3T3 mouse embryonic fibroblast cells. Tenascin immobilized at 0.8 μg/mL, in the presence of 0.1 μg/mL human Fibronectin, will block approximately 55.14% NIH-3T3 cell adhesion (5×10⁴ cells/well, 100 μL/well).

Mouse

HEK293

N-10*His;C-Myc

Q80YX1 (G1884-N2099)

21923  [NCBI]

GLLYPFPRDCSQAMLNGDTTSGLYTIYINGDKTQALEVYCDMTSDGGGWIVFLRRKNGREDFYRNWKAYAAGFGDRREEFWLGLDNLSKITAQGQYELRVDLQDHGESAYAVYDRFSVGDAKSRYKLKVEGYSGTAGDSMNYHNGRSFSTYDKDTDSAITNCALSYKGAFWYKNCHRVNLMGRYGDNNHSQGVNWFHWKGHEYSIQFAEMKLRPSN

27-31 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl,1 mM EDTA, 6% Trehalose, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCL, pH 7.4,10% Glycerol or PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.