TGFBR1/ALK-5 蛋白, Human (sf9, His-GST)

TGFBR1/ALK-5 proteins cooperate with TGFBR2 to form dedicated receptors for TGFB1, TGFB2, and TGFB3, transmitting signals and coordinating different physiological and pathological processes. It induces cell cycle arrest, modulates mesenchymal cell dynamics, contributes to wound healing, and affects immunosuppression and carcinogenesis. TGFBR1/ALK-5 Protein, Human (sf9, His-GST) is the recombinant human-derived TGFBR1/ALK-5 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TGFBR1/ALK-5 Protein, Human (sf9, His-GST) is 304 a.a., with molecular weight of ~57 kDa.

跨膜丝氨酸/苏氨酸激酶 TGFBR1 (ALK-5) 与 TGF-β II 型丝氨酸/苏氨酸激酶受体 TGFBR2 协作，形成 TGF-β 细胞因子的专用受体，包括 TGFB1、TGFB2 和 TGFB3。作为信号转导器，TGFBR1 介导 TGFB1、TGFB2 和 TGFB3 信号从细胞表面到细胞质的传递，从而协调多种生理和病理过程。这些包括上皮细胞和造血细胞的细胞周期停滞、间充质细胞增殖和分化的控制、伤口愈合、细胞外基质的产生、免疫抑制和致癌作用。该受体复合物由 2 个 TGFBR1 和 2 个 TGFBR2 分子对称地与细胞因子二聚体结合组成，导致 TGFBR1 被组成型活性 TGFBR2 磷酸化和激活。激活的 TGFBR1 磷酸化 SMAD2，导致其与受体解离并与 SMAD4 相互作用。由此产生的 SMAD2-SMAD4 复合物易位到细胞核，在细胞核中调节 TGF-β 调节基因的转录，构成典型的 SMAD 依赖性 TGF-β 信号级联。此外，TGFBR1 还参与非典型的、不依赖 SMAD 的 TGF-β 信号通路。例如，它诱导 TRAF6 自动泛素化，导致 MAP3K7 泛素化和激活，引发细胞凋亡。TGFBR1 还通过涉及 PARD6A 磷酸化和激活的 SMAD 独立信号通路来调节上皮细胞向间质细胞的转变。

The specific activity was determined to be > 5 nmol/min/mg using casein as substrate.

Human

Sf9 insect cells

N-His;N-GST

P36897 (T200-M503)

7046  [NCBI]

Approximately 57 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.