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TPBG/5T4 蛋白, Cynomolgus (HEK293, N-His, C-Myc)

目录号: HY-P700467
产品使用指南

TPBG/5T4 蛋白通过与 LRP6 间接相互作用来阻断 Wnt3a 依赖性 LRP6 内化,从而潜在地抑制 Wnt/β-连环蛋白信号传导。这种独特的机制表明 TPBG/5T4 通过破坏 LRP6 内化并影响下游信号传导,在调节 Wnt/β-catenin 通路中发挥着至关重要的作用。TPBG/5T4 蛋白, Cynomolgus (HEK293, N-His, C-Myc) 是重组的 TPBG/5T4 蛋白,由 HEK293 表达,带有 C-Myc, N-10*His 标签。TPBG/5T4 蛋白, Cynomolgus (HEK293, N-His, C-Myc) 全长 321 个氨基酸,分子量为 39.9 kDa。

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描述

TPBG/5T4 protein blocks Wnt3a-dependent LRP6 internalization by indirectly interacting with LRP6, thereby potentially inhibiting Wnt/β-catenin signaling. This unique mechanism suggests that TPBG/5T4 plays a crucial role in regulating the Wnt/β-catenin pathway by disrupting LRP6 internalization and affecting downstream signaling. TPBG/5T4 Protein, Cynomolgus (HEK293, N-His, C-Myc) is the recombinant cynomolgus-derived TPBG/5T4 protein, expressed by HEK293 , with C-Myc, N-10*His labeled tag. The total length of TPBG/5T4 Protein, Cynomolgus (HEK293, N-His, C-Myc) is 321 a.a., with molecular weight of 39.9 kDa.

研究背景

TPBG/5T4 蛋白是 Wnt/β-连环蛋白信号传导的潜在抑制剂,通过与 LRP6 间接相互作用并阻断 Wnt3a 依赖性 LRP6 内化来发挥作用。这种独特的机制表明,TPBG/5T4 通过破坏 LRP6 的内化过程,从而影响下游信号转导事件,在调节 Wnt/β-catenin 通路中发挥着至关重要的作用。对 TPBG/5T4 功能的深入了解强调了其在调节与 Wnt/β-连环蛋白信号传导相关的复杂分子动力学方面的重要性,为细胞调节过程中的治疗探索提供了潜在途径。

种属

Cynomolgus

表达系统

HEK293

标签

C-Myc;N-10*His

蛋白编号

Q4R8Y9 (T35-S355)

基因 ID

102132149  [NCBI]

蛋白结构
N-term
10*His
TLR8 (T35-S355)
Accession # Q4R8Y9
Myc
C-term
同用名
rCynTrophoblast glycoprotein/5T4, His; TPBG; 5T4 Protein, Human; 5T4AG Protein, Human; M6P1 Protein, Human; WAIF1 Protein, Human
氨基酸序列

TSSASSSSSSAPFLASAASAQPPLPDQCPALCECSEAARTVKCVNRNLTEVPTDLPLYVRNLFLTGNQLAVLPAGAFARRPPLAELAALNLSGSRLDEVRGGAFEHLPSLRQLDLSHNPLAYLSPFAFSGSNASISAPSPLVELILNHIVPPDDKRQNRSFEGMVAAALVAGRALQGLHLLELASNHFLYLPRDVLAQLPSLRYLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHVRVFLDNNPWVCDCHMADMVTWLKQTGVVQGKDRLTCAFPEKMRNRVLLELNSADLDCDPILPPSLQTS

分子量

39.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TPBG/5T4 Protein, Cynomolgus (HEK293, N-His, C-Myc) 相关分类

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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TPBG/5T4 Protein, Cynomolgus (HEK293, N-His, C-Myc)
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