1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Trk Receptors TrkA
  5. TrkA 蛋白, Mouse (HEK293, His)

TrkA 蛋白, Mouse (HEK293, His)

目录号: HY-P76116
COA 产品使用指南

TrkA蛋白是一种关键受体酪氨酸激酶,指导神经系统的发育和成熟,影响神经元的增殖、分化和存活。TrkA 是 NGF(其主要配体)的高亲和力受体,并且还对 NTF3/neurotropin-3 做出反应。TrkA 蛋白, Mouse (HEK293, His) 是重组的 TrkA 蛋白,由 HEK293 表达,带有 C-His, C-8*His 标签。TrkA 蛋白, Mouse (HEK293, His) 全长 387 个氨基酸,分子量约为 ~55-125 kDa。

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描述

TrkA protein is a key receptor tyrosine kinase that guides the development and maturation of the nervous system and affects neuronal proliferation, differentiation, and survival. TrkA serves as a high-affinity receptor for NGF (its primary ligand) and also responds to NTF3/neurotropin-3. TrkA Protein, Mouse (HEK293, His) is the recombinant mouse-derived TrkA protein, expressed by HEK293 , with C-His, C-8*His labeled tag. The total length of TrkA Protein, Mouse (HEK293, His) is 387 a.a., with molecular weight of ~55-125 kDa.

研究背景

TrkA 蛋白是一种受体酪氨酸激酶,通过调节交感神经元和感觉神经元的增殖、分化和存活,在中枢和周围神经系统的发育和成熟中发挥着至关重要的作用。它是 NGF 的高亲和力受体,NGF 是其主要配体,也可以被 NTF3/neurotropin-3 激活。虽然NTF3仅通过NTRK1支持轴突延伸,但它对神经元存活没有影响。与二聚体 NGF 配体结合后,TrkA 发生同二聚化、自磷酸化和激活。这种激活导致各种下游效应子(例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1)的募集、磷酸化和/或激活,进而调节驱动细胞存活和分化的不同重叠信号通路。具体来说,TrkA 通过 SHC1 和 FRS2 激活控制细胞分化和存活的 GRB2-Ras-MAPK 级联。此外,TrkA 通过 PLCG1 调节 NF-Kappa-B 激活和细胞存活相关基因的转录。此外,TrkA 还控制由 SHC1 和 SH2B1 介导的 Ras-PI3 激酶-AKT1 信号级联,这进一步有助于细胞存活。在没有配体和激活的情况下,TrkA 可能促进细胞死亡,使神经元的存活依赖于营养因子。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Mouse

表达系统

HEK293

标签

C-8*His

蛋白编号

Q3UFB7/NP_001028296.1 (A34-G420)

基因 ID
蛋白结构
N-term
TrkA (A34-G420)
Accession # Q3UFB7/NP_001028296.1
8*His
C-term
同用名
High affinity nerve growth factor receptor; Trk-A; NTRK1; MTC; TRK
氨基酸序列

ASCREVCCPVGPSGLRCTRAGSLDTLRGLRGAGNLTELYVENQQHLQRLEFEDLQGLGELRSLTIVKSGLRFVAPDAFRFTPRLSHLNLSSNALESLSWKTVQGLSLQDLTLSGNPLHCSCALFWLQRWEQEGLCGVHTQTLHDSGPGDQFLPLGHNTSCGVPTVKIQMPNDSVEVGDDVFLQCQVEGLALQQADWILTELEGAATVKKFGDLPSLGLILVNVTSDLNKKNVTCWAENDVGRAEVSVQVSVSFPASVHLGLAVEQHHWCIPFSVDGQPAPSLRWLFNGSVLNETSFIFTQFLESALTNETMRHGCLRLNQPTHVNNGNYTLLAANPYGQAAASVMAAFMDNPFEFNPEDPIPVSFSPVDGNSTSRDPVEKKDETPFG

分子量

Approximately 55-125 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TrkA Protein, Mouse (HEK293, His)
目录号:
HY-P76116
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