TrkA 蛋白, Rat (HEK293, Fc)

The TrkA protein is a receptor tyrosine kinase that is critical in central and peripheral nervous system development, regulating neuronal proliferation, differentiation, and survival. As a high-affinity NGF receptor, TrkA undergoes homodimerization and autophosphorylation upon ligand binding, activating downstream effectors (SHC1, FRS2, SH2B1, SH2B2, PLCG1) cascade. TrkA Protein, Rat (HEK293, Fc) is the recombinant rat-derived TrkA protein, expressed by HEK293 , with C-hFc labeled tag. The total length of TrkA Protein, Rat (HEK293, Fc) is 418 a.a., with molecular weight of 92-102 kDa.

TrkA 蛋白是一种受体酪氨酸激酶，通过调节交感神经元和感觉神经元的增殖、分化和存活，在中枢和周围神经系统的发育和成熟中发挥着至关重要的作用。作为 NGF（其主要配体）的高亲和力受体，TrkA 也可以被 NTF3/neurotropin-3 激活，尽管 NTF3 通过 NTRK1 特异性支持轴突延伸而不影响神经元存活。在二聚体 NGF 配体结合后，TrkA 经历同二聚化、自磷酸化和激活，随后招募、磷酸化和/或激活下游效应子，例如 SHC1、FRS2、SH2B1、SH2B2 和 PLCG1。这些效应器调节不同但重叠的信号级联，指导细胞存活和分化。通过 SHC1 和 FRS2，TrkA 激活控制细胞分化和存活的 GRB2-Ras-MAPK 级联，而通过 PLCG1，它调节 NF-Kappa-B 激活和对细胞存活至关重要的基因转录。此外，通过 SHC1 和 SH2B1，TrkA 控制 Ras-PI3 激酶-AKT1 信号级联，进一步促进细胞存活的调节。在没有配体和激活的情况下，TrkA 可能促进细胞死亡，强调神经元存活对营养因子的依赖性。

Rat

HEK293

C-hFc

P35739 (M1-P418)

59109  [NCBI]

92-102 kDa

Greater than 95% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.