Tyrosine Hydroxylase 蛋白, Mouse (sf9, His)

The Tyrosine Hydroxylase protein serves as a crucial catalyst in the biosynthesis of catecholamines, including dopamine, noradrenaline, and adrenaline, overseeing the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa)—a pivotal and rate-limiting step in this biochemical pathway. Through the utilization of tetrahydrobiopterin and molecular oxygen, Tyrosine Hydroxylase efficiently transforms tyrosine into L-Dopa, showcasing its central role in the production of essential neurotransmitters. Beyond its primary function, the enzyme exhibits versatility by also catalyzing the hydroxylation of phenylalanine and tryptophan, albeit with lower specificity. Intriguingly, Tyrosine Hydroxylase extends its influence to developmental processes, positively regulating the regression of retinal hyaloid vessels during postnatal development, highlighting its multifaceted engagement in both neurotransmitter synthesis and physiological development. Tyrosine Hydroxylase Protein, Mouse (sf9, His) is the recombinant mouse-derived Tyrosine Hydroxylase protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of Tyrosine Hydroxylase Protein, Mouse (sf9, His) is 497 a.a., with molecular weight of ~65 kDa.

酪氨酸羟化酶蛋白是儿茶酚胺（包括多巴胺、去甲肾上腺素和肾上腺素）生物合成中的关键催化剂，监督 L-酪氨酸向 L-二羟基苯丙氨酸 (L-Dopa) 的转化，这是该生化过程中的关键和限速步骤途径。通过利用四氢生物蝶呤和分子氧，酪氨酸羟化酶有效地将酪氨酸转化为左旋多巴，显示出其在必需神经递质生产中的核心作用。除了其主要功能外，该酶还通过催化苯丙氨酸和色氨酸的羟基化而表现出多功能性，尽管特异性较低。有趣的是，酪氨酸羟化酶将其影响扩展到发育过程，在出生后发育过程中积极调节视网膜玻璃体血管的退化，突显其在神经递质合成和生理发育中的多方面参与。

Mouse

Sf9 insect cells

N-His

P24529 (P2-S498)

21823  [NCBI]

Approximately 65 kDa

Greater than 85% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.