1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. UCHL5 蛋白, Human

UCHL5 蛋白, Human

目录号: HY-P701406
产品使用指南

UCHL5 是一种高度特异性的蛋白酶,作为去泛素化酶与 19S 蛋白酶体亚基错综复杂地连接,裂解“Lys-48”连接的多聚泛素链。尽管 INO80 复合物是惰性的,但 INO80 和蛋白酶体之间的短暂相互作用(由 ADRM1 促进)会激活 UCHL5。UCHL5 蛋白, Human 是重组的 UCHL5 蛋白,由 E. coli 表达,不带标签。UCHL5 蛋白, Human 全长 328 个氨基酸。

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描述

UCHL5 is a highly specific protease that acts as a deubiquitinating enzyme intricately linked to the 19S proteasome subunit, cleaving "Lys-48" linked polyubiquitin chains. Although the INO80 complex is inert, transient interactions between INO80 and the proteasome (facilitated by ADRM1) activate UCHL5. UCHL5 Protein, Human is the recombinant human-derived UCHL5 protein, expressed by E. coli , with tag free. The total length of UCHL5 Protein, Human is 328 a.a., .

研究背景

UCHL5 是一种具有显着特异性的蛋白酶,负责裂解“Lys-48”连接的多聚泛素链,作为与 26S 蛋白酶体的 19S 调节亚基错综复杂地相关的去泛素化酶。虽然 UCHL5 被定位为 INO80 复合体中的假定监管成分,但在此背景下仍然不活跃。然而,在 ADRM1 的促进下,INO80 复合物与蛋白酶体之间的短暂相互作用成为 UCHL5 的关键激活剂,释放其去泛素化能力。这种微妙的调节作用凸显了 UCHL5 参与泛素依赖过程和蛋白酶体机制之间的动态相互作用。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q9Y5K5 (T2-K329)

基因 ID

51377

蛋白结构
N-term
UCHL5 (T2-K329)
Accession # Q9Y5K5
C-term
同用名
UCHL5; Ubiquitin carboxyl-terminal hydrolase isozyme L5; UCH-L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UCHL5 Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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