USP3 蛋白, Human (Active, sf9, GST)

USP3 Protein, Human (Active, sf9, GST) is the recombinant human-derived USP3, expressed by sf9 insect cells , with GST labeled tag. ,

USP3 是一种去泛素化酶，参与多种细胞过程，包括转录调控、细胞周期进程和先天免疫。在 DNA 损伤响应中，USP3 通过去泛素化单泛素化的靶蛋白（如 histone H2A 和 H2AX），拮抗 RNF168 和 RNF8 介导的泛素化，从而促进 DNA 损伤修复因子在 DNA 断裂位点的募集。此外，USP3 是 S 期正常进程和有丝分裂进入所必需的，并通过去泛素化和稳定 TP53 促进正常细胞增殖和转化。在先天免疫记忆的建立中，USP3 通过非转录反馈负调控 TLR 诱导的 NF-kappa-B 信号通路，靶向并移除 MYD88 上的 'Lys-63' 连接的多泛素链。同时，USP3 通过介导 RIGI 和 IFIH1 上的 'Lys-63' 连接的多泛素链负调控 I 型干扰素信号通路的激活。此外，USP3 还去泛素化 ASC/PYCARD（介导大多数炎症小体组装和激活的核心适配蛋白），从而促进炎症小体激活。

The fundamental role of USP3 is specific removal of ubiquitin from substrates. USP3 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP3 used is 1.5 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.

Human

Sf9 insect cells

N-GST

Q9Y6I4-1 (E2-L520)

9960

Partial

Approximately 85.4 kDa

Greater than 90% as determined by reducing SDS-PAGE

溶液

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.