1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. USP53 蛋白, Human (His, GST)

USP53 蛋白, Human (His, GST)

目录号: HY-P701427
产品使用指南

USP53 蛋白是紧密连接中的关键元素,对于听觉毛细胞的存活和听力功能的维持至关重要。尽管缺乏肽酶活性,USP53 的重要性因其与 TJP1:TJP2 异二聚体的相互作用而凸显,这表明 USP53 与紧密连接的分子结构有微妙的关系。这种相互作用强调了 USP53 对细胞存活和听觉过程的潜在影响。USP53 蛋白, Human (His, GST) 是重组的 USP53 蛋白,由 E. coli 表达,带有 N-6*His, N-GST 标签。USP53 蛋白, Human (His, GST) 全长 322 个氨基酸。

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描述

USP53 protein, a critical element in tight junctions, is essential for the survival of auditory hair cells and the maintenance of hearing function. Although lacking peptidase activity, USP53's importance is highlighted by its interaction with the TJP1:TJP2 heterodimer, suggesting nuanced involvement in the molecular architecture of tight junctions. This interaction emphasizes USP53's potential impact on cellular survival and auditory processes. USP53 Protein, Human (His, GST) is the recombinant human-derived USP53 protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of USP53 Protein, Human (His, GST) is 322 a.a., .

研究背景

USP53 蛋白是紧密连接中的重要组成部分,可能通过调节紧密连接屏障特性和机械稳定性,在听毛细胞的存活和听力功能的维持中发挥着至关重要的作用。尽管缺乏肽酶活性,USP53 在听觉功能中的重要性通过其 C 末端区域介导的与异二聚体 TJP1:TJP2 的相互作用得到了强调。这种相互作用表明紧密连接的复杂分子结构有微妙的参与,强调了它对细胞存活和听觉过程的潜在影响。

种属

Human

表达系统

E. coli

标签

N-6*His;N-GST

蛋白编号

Q70EK8 (K30-P351)

基因 ID

54532

蛋白结构
N-term
6*His-GST
USP53 (K30-P351)
Accession # Q70EK8
C-term
同用名
USP53; Inactive ubiquitin carboxyl-terminal hydrolase 53; Inactive ubiquitin-specific peptidase 53
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

USP53 Protein, Human (His, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
USP53 Protein, Human (His, GST)
目录号:
HY-P701427
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