USP8 蛋白, Human (sf9)

USP8 is a key hydrolase in cellular regulation and plays a crucial role in protein turnover by efficiently deubiquitinating proteins and preventing degradation. Its activity spans "Lys-48" and "Lys-63" linked ubiquitin chains, highlighting its versatility. USP8 Protein, Human (sf9) is the recombinant human-derived USP8 protein, expressed by Sf9 insect cells, with tag free.

USP8 是一种在细胞调节中发挥关键作用的水解酶，通过有效去除蛋白质中的缀合泛素，防止其降解，在蛋白质周转中发挥着关键作用。这种酶促作用延伸至“Lys-48”和“Lys-63”连接的泛素链，展示了其在调节泛素动力学方面的多功能性。USP8 的催化活性在 M 期显着增强，强调了其细胞周期依赖性调节功能。该酶错综复杂地参与各种细胞过程，包括细胞增殖和对血清刺激的反应，这是进入 S 期所必需的。此外，USP8 还涉及 T 细胞无反应性的调节、STAM2 和 RASGRF1 等蛋白质的稳定以及内体动力学、货物分类和膜运输的调节。它在控制内体泛素化方面的作用对于维持细胞器的形态至关重要。USP8 进一步对多种途径产生影响，包括顶体生物发生、EGFR 降解、MAPK 信号传导以及 BACE1 介导的 APP 裂解和淀粉样蛋白形成的调节。USP8 的多方面功能凸显了其在维持细胞稳态和协调各种信号级联中的重要性。

The fundamental role of USP8 is specific removal of ubiquitin from substrates. USP8 catalyses the ubiquitin from the substrate Ub-Rho110 to release fluorophores. Rho110 will release 535nM emission light under the excitation condition of 485nM. The signal of which can be quickly and reliably captured using a microplate reader. The concentration of USP8 used is 0.6 nM, and the reaction lasts for 30 minutes. Under these conditions, the signal-to-noise ratio (S/N) of the signal box is greater than or equal to 10.

Human

Sf9 insect cells

Tag Free

P40818-1 (P734-G1110)

9101

Partial

PTVTPTVNRENKPTCYPKAEISRLSASQIRNLNPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEVAEEFGIIMKALWTGQYRYISPKDFKITIGKINDQFAGYSQQDSQELLLFLMDGLHEDLNKADNRKRYKEENNDHLDDFKAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCHKKSRTFEAFMYLSLPLASTSKCTLQDCLRLFSKEEKLTDNNRFYCSHCRARRDSLKKIEIWKLPPVLLVHLKRFSYDGRWKQKLQTSVDFPLENLDLSQYVIGPKNNLKKYNLFSVSNHYGGLDGGHYTAYCKNAARQRWFKFDDHEVSDISVSSVKSSAAYILFYTSLG

43.5 KDa

Greater than 80% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.