YES1 蛋白, Human (sf9, His-GST)

YES1 is a non-receptor protein tyrosine kinase that regulates processes such as cell growth, survival, apoptosis, and cell-cell adhesion. It is activated by RTKs (EGFR, PDGFR, CSF1R, FGFR) to phosphorylate downstream substrates. YES1 Protein, Human (sf9, His-GST) is the recombinant human-derived YES1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of YES1 Protein, Human (sf9, His-GST) is 542 a.a., with molecular weight of ~75 kDa.

YES1 是一种非受体蛋白酪氨酸激酶，是多种细胞过程的关键调节因子，包括细胞生长、存活、凋亡、细胞间粘附、细胞骨架重塑和分化。各种受体酪氨酸激酶 (RTK)（例如 EGFR、PDGFR、CSF1R 和 FGFR）的激活会导致其招募到磷酸化受体，启动下游底物激活和磷酸化。EGFR 激活后，YES1 促进 PARD3 磷酸化，促进上皮紧密连接组装。在细胞与细胞接触时，它会刺激 FYN 和 FER 酪氨酸激酶，导致特定连接成分（如 CTNND1）磷酸化。响应 CXCL12 的 T 细胞刺激，YES1 磷酸化塌陷素反应介导蛋白 2/DPYSL2，诱导 T 细胞迁移。此外，它还参与 CD95L/FASLG 信号传导，介导 AKT 依赖性细胞迁移。YES1 通过细胞周期蛋白依赖性激酶 4/CDK4 的磷酸化、调节 G1 期及其在 G2/M 进程和胞质分裂中的作用，明显参与细胞周期进程。此外，YES1 催化有机阳离子转运蛋白 OCT2 的磷酸化，增强其转运活性。

The specific activity was determined to be > 35 nmol/min/mg using Poly (Glu,Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P07947 (G2-L543)

7525  [NCBI]

Approximately 75 kDa

Greater than 80% as determined by reducing SDS-PAGE

溶液.

Supplied as sterile 20 mM Tris, 500 mM NaCl, 10% gly, 0.5 mM TCEP, pH 8.0

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.