2. Stem Cell/Wnt Apoptosis Cell Cycle/DNA Damage
  3. Deubiquitinase YAP MDM-2/p53 CDK Apoptosis
  4. USP10-IN-4

USP10-IN-4 是一种强效的泛素特异性蛋白酶 10 (USP10) 抑制剂，其 IC50 为 10.87 μM，Kd 为 365 nM。USP10-IN-4 可有效抑制 USP10 介导的下游蛋白 YAP 和 p53 的蛋白酶体降解，从而导致 p53 信号通路中 CDK4 的下调。USP10-IN-4 可促进肝癌细胞凋亡 (apoptosis)，抑制肝癌的发生和发展。USP10-IN-4 可用于肝细胞癌 (HCC) 的研究。

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USP10-IN-4

USP10-IN-4 Chemical Structure

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USP10-IN-4 相关抗体

Top Publications Citing Use of Products

查看 Deubiquitinase 亚型特异性产品:

查看所有亚型
USP1 USP2 USP7 USP8 USP10 USP30 UCHL1

查看 YAP 亚型特异性产品:

查看所有亚型
YAP/TAZ YAP/TAZ-TEAD TEAD YAP1

查看 CDK 亚型特异性产品:

查看所有亚型
CDK1 CDK2 CDK3 CDK4 CDK5 CDK6 CDK7 CDK8 CDK9 CDK11 CDK12 CDK13 CDK14 CDK16 CDK19 CDC CLK Pho85 CDK10 CDK15 CDK17 CDK18 CDK20 PITSLRE

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

USP10-IN-4 is a potent ubiquitin-specific protease 10 (USP10) inhibitor with an IC50 of 10.87 μM and a Kd of 365 nM. USP10-IN-4 effectively inhibits USP10-mediated proteasomal degradation of downstream proteins YAP and p53, leading to the subsequent downregulation of CDK4 in the p53 signaling pathway. USP10-IN-4 promotes apoptosis in HCC cells and inhibits the onset and progression of liver cancer. USP10-IN-4 can used for the study of hepatocellular carcinoma (HCC)[1].

IC50 & Target[1]

CDK4

 

体外研究
(In Vitro)

USP10-IN-4 (Compound LY-2) (0.1-10 μM) 以浓度依赖性方式抑制 Huh-7 和 Bel-7402 细胞的增殖，IC50 分别为 2.5 和 1.3 μM[1]。
USP10-IN-4 (1-20 μM, 14 d) 持续抑制 Huh-7 细胞集落形成[1]。
USP10-IN-4 (5-40 μM, 24 h) 诱导 Huh-7 细胞凋亡和 G1 期阻滞[1]。
USP10-IN-4 (0-20 μM, 0-48 h) 导致 Huh-7 细胞中 YAP、p53、CDK4 和 Cyclin B2 蛋白表达降低[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

USP10-IN-4 相关抗体:

Cell Cycle Analysis[1]

Cell Line: Huh-7 cells
Concentration: 5, 20 and 40 μM
Incubation Time: 24 h
Result: Increased the proportion of cells in the G1 phase from 45% to 68%.

Western Blot Analysis[1]

Cell Line: Huh-7 cells
Concentration: 0, 0.5, 1, 5, 10 and 20 μM
Incubation Time: 0, 6, 12, 24, 36, 48 h
Result: Reduced the expression of YAP and p53 in a time- and concentration-dependent manner.
Reduced CDK4, and Cyclin B2 proteins.
分子量

487.98

Formula

C28H26ClN3O3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

USP10-IN-4 相关分类

  • 摩尔计算器

  • 稀释计算器

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

USP10-IN-4DeubiquitinaseYAPMDM-2/p53CDKApoptosisDUBsYes-associated proteinCyclin dependent kinaseHCCDeubiquitinationBinding affinityCDK4Inhibitorinhibitorinhibit

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USP10-IN-4
目录号：
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