eRF3a 抗体

eRF3a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a.

eRF3a 是 eRF1-eRF3-GTP 三元复合物的 GTPase 组分，该复合物通过识别终止密码子 UAA、UAG 和 UGA 介导翻译终止过程。GSPT1/ERF3A 负责将 ETF1/ERF1 递送至终止密码子：eRF1-eRF3-GTP 复合物与核糖体 A 位点的终止密码子结合，GSPT1/ERF3A 的 GTP 水解诱导构象变化并导致其解离，从而使 ETF1/ERF1 完全占据 A 位点。此外，eRF3a 是瞬时 SURF 复合物的组成部分，在无义介导的 mRNA 降解 (NMD) 过程中招募 UPF1 至停滞的核糖体。它还参与 SHFL 介导的翻译终止，抑制病毒和细胞基因 mRNA 的程序性核糖体移码 (-1PRF)。by similar: 该蛋白的功能在多个研究中被验证，包括复合物结合、构象变化机制以及 NMD 和移码调控中的角色。

P15170

Predicted band size: 55 kDa; Observed band size: 80 kDa

Affinity Purified

Non-conjugated

Unmodified

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1000; IHC: 1:50-1:200

• 
  Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and 293 (lane 4, 15 μg) using eRF3a Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, IHC-P

液体

Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human eRF3a.