1. 抗体
  2. 一抗
  3. 多克隆抗体
  4. eRF3a 抗体

eRF3a Antibody 是一个兔来源、无偶联标记、抗 eRF3a 的 IgG 多克隆抗体。

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规格 价格 是否有货 数量
10 μL ¥700 In-stock
50 μL ¥1750 In-stock
100 μL ¥2800 In-stock
250 μL   询价  

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  • WB: 蛋白质免疫印迹;
  • IHC-P: 石蜡切片样本的免疫组织化学;
  • IHC-F: 冰冻切片样本的免疫组织化学;
  • ICC/IF: 细胞免疫荧光;
  • IF-Tissue: 组织免疫荧光;
  • mIHC: 多重荧光免疫组化;
  • IP: 免疫沉淀;
  • ChIP: 染色质免疫沉淀;
  • FC: 流式细胞术;
  • ELISA: 酶联免疫吸附试验
  • 产品详情

  • 验证图片

  • 背景信息

  • 产品资料

描述

eRF3a Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to eRF3a.

宿主

Rabbit

克隆性

Polyclonal

分子量
Predicted band size: 55 kDa;
Observed band size: 80 kDa
请注意:因蛋白存在修饰或聚体等情况,以实测为准,预测仅为参考。
反应种属
Human, Mouse, Rat
蛋白数据库
基因 ID
免疫原

Synthetic peptide corresponding to Human eRF3a.

应用 & 推荐
稀释比例
应用 稀释比
WB
WB: 蛋白质免疫印迹
1:500-1:1000
IHC-P
IHC-P: 石蜡切片样本的免疫组织化学
1:50-1:200
性状

液体

组分

Supplied in PBS with 0.02% sodium azide, 50% glycerol, pH7.2.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

验证图片
WB
  • Western blot analysis was performed on extracts from HepG2 (lane 1, 15 μg), Hela (lane 2, 15 μg), 3T3 (lane 3, 15 μg), and 293 (lane 4, 15 μg) using eRF3a Rabbit pAb.Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Tubulin, HY-P80487, 1:60000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti-Rabbit IgG-HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

同用名
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog; GSPT1; ERF3A
文件资料

eRF3a Antibody 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
eRF3a Antibody
目录号:
HY-P83766
需求量: