JAK3 抗体 (YA2466)

JAK3 Antibody (YA2466) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to JAK3.

The protein encoded by The related gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in The related gene are associated with autosomal SCID (severe combined immunodeficiency disease).

P52333

Predicted band size: 125 kDa; Observed band size: 125 kDa

Affinity Chromatography

Non-conjugated

Unmodified

AB_3104820

Signal Transduction

Primary Antibody; Recombinant Rabbit Monoclonal Antibody

Recombinant, Monoclonal

Rabbit

Human

WB: 1:500-1:1000; IF: 1:50-1:200

• 
  Western blot analysis was performed on extracts from Jurkat (lane 1, 20 μg),using JAK3 Rabbit mAb.Proteins were transferred to a PVDF membrane and blocked with 5% non - fat milk in TBST at 4°C overnight.The primary antibody (1:1000 dilution) and the loading control antibody (beta-Actin, HY-P83730, 1:5000 dilution) were incubated in 5% non-fat milk in TBST for 1 hour at 37°C.Goat Anti - Mouse/Rabbit IgG - HRP Secondary Antibody (1:20000 dilution) was then applied for 40 minutes at 37°C.

WB, ICC/IF

液体

Supplied in 50 mM Tris-Glycine(pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

A synthesized peptide derived from human JAK3