TFEB 抗体 (YA5545)

TFEB Antibody (YA5545) is a mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to TFEB. It can be applicated for IHC-P, WB, ICC/IF, ELISA assays, in the background of human, mouse.

TFEB 蛋白作为关键转录因子协调多种细胞过程，包括溶酶体生物发生、自噬、溶酶体胞吐作用、脂质分解代谢、能量代谢和免疫反应。识别 E-box 序列后，TFEB 的有效 DNA 结合需要与其自身或其他 MiT/TFE 家族成员（如 TFE3 或 MITF）进行二聚化。它通过 MTOR 介导的磷酸化对氨基酸可用性做出反应，促进细胞质保留和失活。相反，饥饿或溶酶体应激期间的 MTOR 抑制会诱导 TFEB 去磷酸化，从而导致核定位和转录激活。TFEB 特异性结合 CLEAR-box 序列，激活溶酶体基因的表达并调节溶酶体定位。此外，它还能积极调节自噬，促进 T 细胞依赖性抗体反应，并在胎盘血管化中发挥作用。为了应对细菌感染，TFEB 介导衣康酸诱导的烷基化，激活溶酶体生物发生并帮助细菌清除。值得注意的是，艾曲波帕药物通过与 bHLH 结构域结合并破坏 DNA 结合来抑制 TFEB。

P19484

Predicted band size: 53 kDa; Observed band size: 50 kDa

Protein G

Non-conjugated

Unmodified

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse,

IHC: 1:200-1000; WB: 1:500-2000; ICC/IF: 1:100-500; ELISA: 1:1000-5000

IHC-P, WB, ICC/IF, ELISA

液体

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Synthesized peptide derived from human TFEB AA range: 300-440